Host: Rabbit
Target Protein: NF-H
Immunogen Range: 21-120/1026
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4744
Swiss Prot: P12036
Source: KLH conjugated synthetic peptide derived from human NF-H
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 118
Cross Reactive Species:
Human
Rat
Chicken
Predicted Cross Reactive Species:
Mouse
Dog
Cow
Sheep
Pig
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Liu, Yi, et al. "Conserved Dopamine Neurotrophic Factor-Transduced Mesenchymal Stem Cells Promote Axon Regeneration and Functional Recovery of Injured Sciatic Nerve." PLOS ONE 9.10 (2014): e110993.Read more>>
- Liu, Xin-Qi, et al. "Regulation of neuroendocrine cells and neuron factors in the ovary by zinc oxide nanoparticles." Toxicology Letters (2016).Read more>>
- Lu et al. Autophagy activator promotes neuronal differentiation of adult adipose-derived stromal cells. (2013) Neural.Regen.Res. 8:882-9Read more>>
- Zhang Heng. et al. Bone Morphogenetic Protein-7 (BMP-7) Promotes Neuronal Differentiation of Bone Marrow Mesenchymal Stem Cells (BMSCs) In Vitro. Biomed Res Int. 2021;2021:7239783Read more>>
- Injoo Hwang. et al. Endothelin-1 enhances the regenerative capability of human bone marrow-derived mesenchymal stem cells in a sciatic nerve injury mouse model. Biomaterials. 2021 Aug;275:12098Read more>>
Formalin-fixed and paraffin embedded rat brain labeled with Anti-NF-H Polyclonal Antibody, Unconjugated (bs-10680R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-NF-H Polyclonal Antibody, Unconjugated (bs-10680R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
U-87MG lysates probed with NF-H Polyclonal Antibody, Unconjugated (bs-10680R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Tissue/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (NF-H) polyclonal Antibody, Unconjugated (bs-10680R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.