bs-10666R-Cy5.5

DATASHEET

Host: Rabbit

Target Protein: AMPK alpha-1(Ser184)

Specificity: Based on the high protein similarity this antibody will bind AMPK alpha 1 when phorphorylated at Ser184 and AMPK alpha 2 when phosphrylated at Ser173.

Immunogen Range: 170-200/559

Modification Site: Ser184

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5562

Swiss Prot: Q13131

Source: KLH conjugated Synthesised phosphopeptide derived from human AMPK alpha 1 around the phosphorylation site of Ser184

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008].

Conjugation: Cy5.5

Excitation/ Emission: 675nm/694nm

Size: 100ul

Concentration: 1ug/ul

Applications: IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 64 kDa

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.