DATASHEET
Host:
Rabbit
Target Protein:
Troponin T
Immunogen Range:
201-298/298
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
7139
Swiss Prot:
P45379
Source:
KLH conjugated synthetic peptide derived from human Cardiac Troponin T
Purification:
Purified by Protein A.
Storage Buffer:
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Conjugation:
FITC
Excitation/ Emission:
494nm/518nm
Size:
100ul
Concentration:
1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
36
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zhao et al. Rho-associated protein kinases play an important role in the differentiation of rat adipose-derived stromal cells into cardiomyocytes in vitro. (2014) PLoS.One. 9:e115191Read more>>
- Jia, Li. et al. A Novel Splicing Mutation c.3351 G_>_A in the Cardiac Transcription Factor NKX2-5 Leads to Familial Atrial Septal Defect Through miR-19 and PYK2. Stem Cell Reviews and Reports. 2022 Jul;:1-16Read more>>
- Wenhao Han. et al. Targeting Myocardial Mitochondria-STING-Polyamine Axis Prevents Cardiac Hypertrophy in Chronic Kidney Disease. JACC-BASIC TRANSL SC. 2022 AugRead more>>
- Li Chen. et al. l-Arginine and l-lysine can weaken the intermolecular interactions of main myofibrillar proteins: the roles in improving the tenderness of pork Longissimus lumborum muscle. INT J FOOD SCI TECH. 2023 ApRead more>>
- Dr. Rabia Onbas. et al. Biopatterning of 3D Cellular Model by Contactless Magnetic Manipulation for Cardiotoxicity Screening. Tissue Engineering Part A. 2023 Nov 17Read more>>
- Lai Yiwei. et al. Multimodal cell atlas of the ageing human skeletal muscle. NATURE. 2024 Apr;629(8010):154-164Read more>>
- Wang Ya. et al. miR-29b-3p regulates cardiomyocytes pyroptosis in CVB3-induced myocarditis through targeting DNMT3A. CELL MOL BIOL LETT. 2024 Dec;29(1):1-2Read more>>
- Mengni Bao. et al. PICALM Regulating the Generation of Amyloid -Peptide to Promote Anthracycline-Induced Cardiotoxicity. ADV SCI. 2024 Jun;:2401945Read more>>
VALIDATION IMAGES
U2-OS cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Troponin T Polyclonal Antibody(bs-10648R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
U2-OS cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Troponin T Polyclonal Antibody(bs-10648R-FITC)at 1:50dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).