bs-1062R [Primary Antibody]
ADRA2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ADRA2

Immunogen Range: 385-450/450


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 150

Swiss Prot: P08913

Source: KLH conjugated synthetic peptide derived from human ADRA2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 50


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sun Y et al. Changes in TRPV1 expression in the POA of ovariectomized rats regulated by NE-dependent 2-ADR may be involved in hot flashes. Ann Anat. 2020 Nov;232:151565.Read more>>
  • Yanrong Sunet al. Changes in TRPV1 expression in the POA of ovariectomized rats regulated by NE-dependent alpha 2-ADR may be involved in hot flashes. Ann Anat. 2020 Nov;232:151565.Read more>>
  • Shunsuke Kawanabe. et al. Upregulations of 1 adrenergic receptors and noradrenaline synthases in the medial prefrontal cortex are associated with emotional and cognitive dysregulation induced by post-weaning social isolation in male rats. NEUROSCI LETT. 2023 Feb;797:137071Read more>>
VALIDATION IMAGES

Formalin-fixed and rat brain tissue labeled with Anti-ADRA2 Polyclonal Antibody, Unconjugated (bs-1062R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


L1 rat kidney lysates, L2 rat brain lysates probed (bs-1062R) Anti-ADRA2 Polyclonal, Unconjugated at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 50kD.