Host: Rabbit
Target Protein: Integrin Alpha 3 + Beta 1
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3675, 3688
Swiss Prot: P26006, P05556
Source: Mixed KLH conjugated synthetic peptide derived from human Integrin Alpha 3(976-1025/1051) + Beta 1(35-47/798)
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 115
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
- Wang, G., et al. "PAK1 regulates RUFY3-mediated gastric cancer cell migration and invasion." Cell Death & Disease 6.3 (2015): e1682.Read more>>
- Zhang C et al. CXCR4-Overexpressing Umbilical Cord Mesenchymal Stem Cells Enhance Protection against Radiation-Induced Lung Injury. Stem Cells Int. 2019 Feb 5;2019:2457082. Read more>>
- Li R et al. Metabolomic analysis reveals metabolic alterations of human peripheral blood lymphocytes by perfluorooctanoic acid. Chemosphere. 2019 Sep 7;239:124810. Read more>>
- Derya Karabulut. et al. A different perspective on the filtration barrier after kidney stone formation: An immunohistochemical and biochemical study. 2020 Nov 05Read more>>
- Anqi Liu. et al. Core fucosylation involvement in the paracrine regulation of proteinuria-induced renal interstitial fibrosis evaluated with the use of a microfluidic chip. Acta Biomater. 2022 FebRead more>>
- Xu, Jun. et al. Mutation of Beclin1 acetylation site at K414 alleviates high glucose-induced podocyte impairment in the early stage of diabetic nephropathy by inhibiting hyperactivated autophagy. Mol Biol Rep. 2022 Feb;:1-8Read more>>
- Xiaoyin Guo. et al. Mechanism underlying the role of integrin 31 in adhesive dysfunction between thyroid cells induced by diesel engine exhaust particles. SCI TOTAL ENVIRON. 2024 Oct;947:174535Read more>>
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Integrin Alpha 3 + Beta 1 Polyclonal Antibody (bs-1057R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-Integrin Alpha 3 + Beta 1 Polyclonal Antibody (bs-1057R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Formalin-fixed and paraffin embedded rat pancreas labeled with Rabbit Anti Integrin Alpha 3 + Beta 1 Polyclonal Antibody, Unconjugated (bs-1057R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Rabbit Anti-Integrin Alpha 3 + Beta 1 Polyclonal Antibody, Unconjugated (bs-1057R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.
A431 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin Alpha 3 + Beta 1 Antibody(bs-1057R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).