Host: Rabbit
Target Protein: Connexin-40
Immunogen Range: 301-358/358
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2702
Swiss Prot: P36382
Source: KLH conjugated synthetic peptide derived from human Connexin-40
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 40
Cross Reactive Species:
Mouse
Rat
Dog
Predicted Cross Reactive Species:
Cow
Sheep
Pig
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Zhao, Jing, et al. "Chronic obstructive sleep apnea causes atrial remodeling in canines: mechanisms and implications." Basic Research in Cardiology 109.5 (2014): 1-13.Read more>>
- Wang, Yu, et al. "Tanshinone II A Relieves Adriamycin-induced Myocardial Injury in Rat Model." International Journal of Chemistry 8.1 (2016): 40.Read more>>
- Zhangchi Liu. et al. Pinocembrin alleviates the susceptibility to atrial fibrillation in isoproterenol-induced rats. BIOCHEM BIOPH RES CO. 2022 Dec;636:33Read more>>
- Hu Junyi. et al. Multi-omic profiling of clear cell renal cell carcinoma identifies metabolic reprogramming associated with disease progression. NAT GENET. 2024 Feb;56(3):442-457Read more>>
Formalin-fixed and paraffin embedded rat myocardium tissue labeled with Anti-Connexin-40 Polyclonal Antibody, Unconjugated (bs-1050R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse spleen cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Connexin-40 Polyclonal Antibody(bs-1050R-AF488)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Kidney Lysates; Lane 2: Rat Kidney Lysates. Probed with Connexin-40 polyclonal Antibody, unconjugated (bs-1050R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Connexin-40 Polyclonal Antibody, Unconjugated (bs-1050R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Connexin-40 Polyclonal Antibody, Unconjugated (bs-1050R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Connexin-40 Polyclonal Antibody, Unconjugated (bs-1050R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with Connexin-40 polyclonal antibody, unconjugated (bs-1050R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.