bs-10472R [Primary Antibody]
TLR2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TLR2

Immunogen Range: 301-400/784


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7097

Swiss Prot: O60603

Source: KLH conjugated synthetic peptide derived from human TLR2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Predicted Molecular Weight: 84


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Jihong, et al. "Toll-Like Receptor 2 Deficiency Shifts PrP106-126-Induced Microglial Activation from a Neurotoxic to a Neuroprotective Phenotype." Journal of Molecular Neuroscience (2014): 1-11.Read more>>
  • Wang, Shaolan, et al. "Xenobiotic Pregnane X Receptor (PXR) Regulates Innate Immunity via Activation of NLRP3 Inflammasome in Vascular Endothelial Cells." Journal of Biological Chemistry 289.43 (2014): 30075-30081.Read more>>
  • Fu, Juanli, et al. "Tetrachlorobenzoquinone exerts neurological pro-inflammatory activity by promoting HMGB1 release, which induces TLR4 clustering within the lipid raft." Toxicological Sciences (2016): kfw124.Read more>>
  • Silvestro Ennio D'Anna. et al. Bacterial load and related innate immune response in the bronchi of rapid decliners with chronic obstructive pulmonary disease. RESP MED. 2023 Aug;215:107297Read more>>
  • Ni Shuangfei. et al. METTL3 promotes nucleus pulposus cell senescence in intervertebral disc degeneration by regulating TLR2 m6A methylation and gut microbiota. J GERONTOL A-BIOL. 2024 JuRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-TLR2 Polyclonal Antibody, Unconjugated (bs-10472R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Rat Spleen tissue lysates; Lane 2: Rat Bronchus tissue lysates; Lane 3: Rat Lung tissue Lysates probed with TLR2 Polyclonal Antibody, Unconjugated (bs-10472R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.