bs-1044R [Primary Antibody]
MIF Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MIF

Immunogen Range: 51-115/115


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4282

Swiss Prot: P14174

Source: KLH conjugated synthetic peptide derived from human MIF

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 12


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Hiress et al. Proinflammatory Signature of the Dysfunctional Endothelium in Pulmonary Hypertension. Role of the Macrophage Migration Inhibitory Factor/CD74 Complex. (2015) Am.J.Respir.Crit.Care.Med. 192:983-97Read more>>
  • Xu Q et al. Expression of macrophage migration inhibitory factor in Aspergillus fumigatus keratitis. Int J Ophthalmol. 2019 May 18;12(5):711-716. Read more>>
  • Hou J et al. The expression of macrophage migration inhibitory factor and intercellular adhesion molecule-1 in rats with periodontitis and atherosclerosis. Arch Oral Biol. 2019 Aug 10;107:104513. Read more>>
  • Jang Jaemyung. et al. Abnormal accumulation of extracellular vesicles in hippocampal dystrophic axons and regulation by the primary cilia in Alzheimers disease. ACTA NEUROPATHOL COM. 2023 Dec;11(1):1-3Read more>>
  • Liu Junhao. et al. Tanshinol ameliorates imiquimod-induced psoriasis by inhibiting M1 macrophage polarization through suppression of the notch signaling pathway. N-S ARCH PHARMACOL. 2024 Jun;:1-14Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat skin labeled with Anti-MIF Polyclonal Antibody, Unconjugated (bs-1044R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded human glioma labeled with Anti-MIF Polyclonal Antibody, Unconjugated (bs-1044R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Lane 1: Rat Aldrenal gland Lysates; Lane 2: Human HL-60 cell Lysates. Probed with MIF polyclonal Antibody, unconjugated (bs-1044R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.