bs-10446R-PE [Conjugated Primary Antibody]
HPV16-E7 Polyclonal Antibody, PE Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HPV16-E7

Specificity: This antibody may also detect HPV35-E7

Immunogen Range: 21-98/98


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1489079

Swiss Prot: P03129

Source: KLH conjugated synthetic peptide derived from middle of HPV16-E7

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Plays also a role in the inhibition of both antiviral and antiproliferative functions of host inteferon alpha.

Conjugation: PE

Excitation/ Emission: 496,564nm/578nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)

Predicted Molecular Weight: 11


Cross Reactive Species: Human
Virus
Others
(HPV16)

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shao, Jian-Shuang, et al. "HPV16 E6/E7 upregulates HIF-2α and VEGF by inhibiting LKB1 in lung cancer cells." Tumor Biology 39.7 (2017): 1010428317717137.Read more>>
  • Liu, Yuan, et al. "Targeting hexokinase 2 inhibition promotes radiosensitization in HPV16 E7-induced cervical cancer and suppresses tumor growth." International Journal of Oncology 50.6 (2017): 2011-2023.Read more>>
  • Yang JH et al. HPV16 E6/E7 upregulate hTERC mRNA and gene amplification levels by relieving the effect of LKB1 on Sp1 phosphorylation in lung cancer cells:. Ther Adv Med Oncol.2020 May 12;12:1758835920917562.Read more>>
  • Koji K et al. Evaluation of HPV16 E7 expression in head and neck carcinoma cell lines and clinical specimensSci Rep.2020 Dec 17;10(1):22138.Read more>>
  • Paola Matarrese. et al. Physical Interaction between HPV16E7 and the Actin-Binding Protein Gelsolin Regulates Epithelial-Mesenchymal Transition via HIPPO-YAP Axis. Cancers. 2021 Jan;13(2):353Read more>>
  • Prabakaran D. S.. et al. Fused toes homolog, a potential molecular regulator of human papillomavirus type 16 E6 and E7 oncoproteins in cervical cancer. PLOS ONE. 2022 Apr;17(4):e0266532Read more>>
VALIDATION IMAGES