bs-10442R
[Primary Antibody]
Caspase-1 p20 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Caspase-1 p20
Immunogen Range: 181-280/402
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 12362
Swiss Prot: P29452
Source: KLH conjugated synthetic peptide derived from mouse Caspase-1 p20
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 20/46
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Chen JW et al. Zhihuang Tongfeng decoction ameliorates gouty arthritis via inhibition of NLRP3 inflammasome in rats. Tropical Journal of Pharmaceutical Research December 2019; 18 (12): 2577-2582.Read more>>
- Chen JW et al. Zhihuang Tongfeng decoction ameliorates gouty arthritis via inhibition of NLRP3 inflammasome in rats. Tropical Journal of Pharmaceutical Research December 2019; 18 (12): 2577-2582.Read more>>
- Hanwen Li et al. P2Y 14 receptor has a critical role in acute gouty arthritis by regulating pyroptosis of macrophages. Cell Death Dis.2020 May 26;11(5):394.Read more>>
- Xiping Yang. et al. The piperine derivative HJ105 inhibits A1-42-induced neuroinflammation and oxidative damage via the Keap1-Nrf2-TXNIP axis. Phytomedicine. 2021 Apr;:153571Read more>>
- You Zhou. et al. Overexpression of lncRNA TUG1 Alleviates NLRP3 Inflammasome-Mediated Cardiomyocyte Pyroptosis Through Targeting the miR-186-5p/XIAP Axis in Coronary Microembolization-Induced Myocardial Damage. Front Immunol. 2021; 12: 637598Read more>>
- Jia-Jia Xu. et al. Anti-inflammatory effect of a polysaccharide fraction from Craterellus cornucopioides in LPS-stimulated macrophages. 2021 Jun 3Read more>>
- Hongli Guo. et al. Transcriptional enhancement of GBP-5 by BATF aggravates sepsis-associated liver injury via NLRP3 inflammasome activation. Faseb J. 2021 Jun;35(6):e21672Read more>>
- Chenyu Zhang. et al. The Nrf2-NLRP3-caspase-1 axis mediates the neuroprotective effects of Celastrol in Parkinson's disease. Redox Biol. 2021 Nov;47:102134Read more>>
- Jie Ma. et al. The Effect of MaxiK Channel on Regulating the Activation of NLRP3 Inflammasome in Rats of Blast-induced Traumatic Brain Injury. Neuroscience. 2022 Feb;482:132Read more>>
- Xianglin Pan. et al. Pectic polysaccharide from Smilax china L. ameliorated ulcerative colitis by inhibiting the galectin-3/NLRP3 inflammasome pathway. Carbohyd Polym. 2022 Feb;277:118864Read more>>
- Meiyun Cai. et al. Kaemperfol alleviates pyroptosis and microglia-mediated neuroinflammation in Parkinson's disease via inhibiting p38MAPK/NF-B signaling pathway. Neurochem Int. 2021 Nov;:105221Read more>>
- Matz Isabell. et al. Left ventricle- and skeletal muscle-derived fibroblasts exhibit a differential inflammatory and metabolic responsiveness to interleukin-6. FRONT IMMUNOL. 2022 Jul;0:4049Read more>>
- Shuai Jiang. et al. Cardiac-specific overexpression of Claudin-5 exerts protection against myocardial ischemia and reperfusion injury. BBA-MOL BASIS DIS. 2022 Sep;:166535Read more>>
- Xu Yang. et al. T-2 Toxin Caused Mice Testicular Inflammation Injury via ROS-Mediated NLRP3 Inflammasome Activation. J AGR FOOD CHEM. 2022;XXXX(XXX):XXX-XXXRead more>>
- Guo-Ping Wang. et al. To Analyze the Mechanism of SalB Regulating SIRT1 to Inhibit NLRP3 and Its Ameliorative Effect on Tubulogastric Junction Tumor Lesions Complicated with Myocardial Injury. BIOMED RES INT. 2022 Oct 14;2022:6560693Read more>>
- Yani He. et al. Glycolytic reprogramming controls periodontitis-associated macrophage pyroptosis via AMPK/SIRT1/NF-B signaling pathway. INT IMMUNOPHARMACOL. 2023 Jun;119:110192Read more>>
- Ding Huiru. et al. Dihydromyricetin Alleviates Ischemic Brain Injury by Antagonizing Pyroptosis in Rats. NEUROTHERAPEUTICS. 2023 Aug;:1-12Read more>>
- Kun Lin Zhou. et al. IL-17A/p38 Signaling Pathway Induces Alveolar Epithelial Cell Pyroptosis and Hyperpermeability in Sepsis-Induced Acute Lung Injury by Activating NLRP3 Inflammasome. Advanced Biology. 2023 Aug;:230022Read more>>
- Liu Chunxiao. et al. Targeting P2Y14R protects against necroptosis of intestinal epithelial cells through PKA/CREB/RIPK1 axis in ulcerative colitis. NAT COMMUN. 2024 Mar;15(1):1-16Read more>>
- Hao Li. et al. Polysaccharides from an edible mushroom, Hericium erinaceus, alleviate ulcerative colitis in mice by inhibiting the NLRP3 inflammasomes and reestablish intestinal homeostasis. INT J BIOL MACROMOL. 2024 Mar;:131251Read more>>
- Anjun Song. et al. An Alkaline Nanocage Continuously Activates Inflammasomes by Disrupting Multiorganelle Homeostasis for Efficient Pyroptosis. ACS APPL MATER INTER. 2024;16(19):2429524307Read more>>
- Qifang Sun. et al. Astragaloside IV ameliorates indomethacin-induced intestinal inflammation in rats through inhibiting the activation of NLRP3 inflammasome. INT IMMUNOPHARMACOL. 2024 Jun;135:112281Read more>>
- Mei Zhang. et al. Protective Effects of Resveratrol on Cytotoxicity of Mouse Hepatic Stellate Cells Induced by PM2.5. ATMOSPHERE-BASEL. 2024 May;15(5):588Read more>>
- Chengzhi Yuan. et al. Atractylenolide I Alleviates Indomethacin-Induced Gastric Ulcers in Rats by Inhibiting NLRP3 Inflammasome Activation. J AGR FOOD CHEM. 2024;XXXX(XXX):XXX-XXXRead more>>
VALIDATION IMAGES
Rat lung lysates probed with Caspase-1 p20 Polyclonal Antibody, Unconjugated (bs-10442R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Caspase-1 p20 Polyclonal Antibody(bs-10442R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Heart lysates; Lane 2: Rat Heart lysates probed with Caspase-1 p20 Polyclonal Antibody, Unconjugated (bs-10442R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.