DATASHEET
Host:
Rabbit
Target Protein:
C2a
Immunogen Range:
401-500/752
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
717
Swiss Prot:
P06681
Source:
KLH conjugated synthetic peptide derived from human C2a
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor C4b to generate the C3 or C5 convertase.
PRODUCT SPECIFIC PUBLICATIONS
- Caine, Jennifer A., et al. "Borrelia burgdorferi outer surface protein C (OspC) binds complement component C4b and confers bloodstream survival." Cellular Microbiology (2017).Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (PDHA1) Polyclonal Antibody, Unconjugated (bs-4034R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (C2a ) Polyclonal Antibody, Unconjugated (bs-10428R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.