bs-10403R
[Primary Antibody]
Pan Cytokeratin/p-CK Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Pan Cytokeratin/p-CK
Clonality: Polyclonal
Isotype: IgG
Source: KLH conjugated synthetic peptide derived from human cytokeratin 16
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Antibody to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-2000)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)
Flow-Cyt(Flow-Cyt=1μg /test)
ICC/IF(ICC/IF=1:100)
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Niu YN, Wang K, Jin S, Fan DD, Wang MS, Xing NZ, Xia SJ. "The intriguing role of fibroblasts and c-Jun in the chemopreventive and therapeutic effect of finasteride on xenograft models of prostate cancer." Asian J AndrolRead more>>
- Napolitano Stefania. et al. Antitumor efficacy of dual blockade with encorafenib plus cetuximab in combination with chemotherapy in human BRAFV600E mutant colorectal cancer.. CLIN CANCER RES. 2023 ApRead more>>
- Hiroshi Fukushima. et al. Phototruncation cell tracking with near-infrared photoimmunotherapy using heptamethine cyanine dye to visualise migratory dynamics of immune cells. EBIOMEDICINE. 2024 Apr;102:10505Read more>>
- Lixiang Zhao. et al. Dicliptera chinensis -derived polysaccharide enhanced the growth activity of submandibular gland cells in vitro after radiotherapy. HELIYON. 2024 09Read more>>
- Hiroshi Fukushima. et al. Near-infrared duocarmycin photorelease from a Treg-targeted antibody-drug conjugate improves efficacy of PD-1 blockade in syngeneic murine tumor models. ONCOIMMUNOLOGY. 2024 2Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded mouse lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Pan cytokeratin Polyclonal Antibody, Unconjugated (bs-10403R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Pan cytokeratin Polyclonal Antibody, Unconjugated (bs-10403R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAPI staining
Paraformaldehyde-fixed, paraffin embedded mouse lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Pan cytokeratin Polyclonal Antibody, Unconjugated (bs-10403R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin/p-CK) Polyclonal Antibody, Unconjugated (bs-10403R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin/p-CK) Polyclonal Antibody, Unconjugated (bs-10403R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin/p-CK) Polyclonal Antibody, Unconjugated (bs-10403R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Human Hela cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human A673 cell lysates probed with Pan Cytokeratin Polyclonal Antibody, Unconjugated (bs-10403R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.