Host: Rabbit
Target Protein: Alpha-actinin-2
Immunogen Range: 351-450/894
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 88
Swiss Prot: P35609
Source: KLH conjugated synthetic peptide derived from human Sarcomeric Alpha Actinin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 98
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species:
Human
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Liu, Yaowen, Shuyao Wang, and Rong Zhang. "Composite poly (lactic acid)/chitosan nanofibrous scaffolds for cardiac tissue engineering." International Journal of Biological Macromolecules (2017).Read more>>
- Liu Y et al. Ghrelin protects the myocardium with hypoxia/reoxygenation treatment through upregulating the expression of growth hormone, growth hormone secretagogue receptor and insulin-like growth factor-1, and promoting the phosphorylation of protein kinase B. Int. J. Mol. Med. Sep 18Read more>>
- Liu et al. Ghrelin protects the myocardium with hypoxia/reoxygenation treatment through upregulating the expression of growth hormone, growth hormone secretagogue receptor and insulin-like growth factor-1, and promoting the phosphorylation of protein kinase B. (2018) Int.J.Mol.Med. 42:3037-3046Read more>>
- Yang Juesheng. et al. Protective effect of inhibiting TRPM7 expression on hypoxia post-treatment H9C2 cardiomyocytes. Clin Hemorheol Micro. 2021 Jan;77(1):91-105Read more>>
- Yi Shen. et al. Pressure overload promotes cystatin C secretion of cardiomyocytes to regulate the MAPK signaling pathway and mediate cardiac hypertrophy. Ann Transl Med. 2020 Nov; 8(22): 1514Read more>>
- Yuan Wu. et al. Carotid baroreceptor stimulation prevents oxidative stress by inhibiting MAO-A and prevents cardiac remodeling in obese rats. OBESITY. 2023 MaRead more>>
Formalin-fixed and paraffin embedded rat heart labeled with Rabbit Anti-Sarcomeric Alpha Actinin Polyclonal Antibody, Unconjugated (bs-10367R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Sarcomeric Alpha Actinin) Polyclonal Antibody, Unconjugated (bs-10367R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.