bs-1035R [Primary Antibody]
CD86 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD86

Immunogen Range: 140-175/313


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 56822

Swiss Prot: O35531

Source: KLH conjugated synthetic peptide derived from rat CD86

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 31


Cross Reactive Species: Human
Mouse
Rat
Dog

Predicted Cross Reactive Species: Cow
Sheep
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhou, Xi-Jin, et al. "Vasoactive Intestinal Peptide Promotes Immune Escape of MKN45 Cells by Inhibiting Antigen-Presenting Molecules of Dendritic Cells In Vitro." International Journal of Peptide Research and Therapeutics: 1-13.Read more>>
  • Xie, Juan, et al. "PICK1 confers anti-inflammatory effects in acute liver injury via suppressing M1 macrophage polarization." Biochimie (2016).Read more>>
  • Chen, Quan, et al. "Prevention of ultraviolet radiation‑induced immunosuppression by sunscreen in Candida albicans‑induced delayed‑type hypersensitivity." Molecular Medicine Reports (2016).Read more>>
  • Das, Subhamoy, et al. "Syndesome Therapeutics for Enhancing Diabetic Wound Healing." Advanced Healthcare Materials (2016).Read more>>
  • Ju, Huanyu, et al. "An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model." BMC immunology 17.1 (2016): 31.Read more>>
  • Liang, Zhuoping, et al. "Role of dendritic cells in consistency between upper and lower airway inflammatory responses: animal experimental studies." Int J Clin Exp Pathol 9.10 (2016): 10093-10104.Read more>>
  • Zhang, Suxin, et al. "Variation and significance of secretory immunoglobulin A, interleukin 6 and dendritic cells in oral cancer." Oncology Letters.Read more>>
  • Chen, Xionglin, et al. "Peptide-modified chitosan hydrogels promote skin wound healing by enhancing wound angiogenesis and inhibiting inflammation." Am J Transl Res 9.5 (2017): 2352-2362.Read more>>
  • Han, Sheng, et al. "LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4." Journal of Experimental & Clinical Cancer Research 36.1 (2017): 83.Read more>>
  • Gerzanich, Volodymyr, et al. "Salutary effects of glibenclamide during the chronic phase of murine experimental autoimmune encephalomyelitis." Journal of Neuroinflammation 14.1 (2017): 177.Read more>>
  • Gao et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. (2013) Cell.Transplan. 22:1883-900Read more>>
  • Chen et al. Mesenchymal stem cell-laden anti-inflammatory hydrogel enhances diabetic wound healing. (2015) Sci.Re. 5:18104Read more>>
  • Ju et al. An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model. (2016) BMC.Immunol. 17:31Read more>>
  • Matsui et al. M1 Macrophages Are Predominantly Recruited to the Major Pelvic Ganglion of the Rat Following Cavernous Nerve Injury. (2017) J.Sex.Med. 14:187-195Read more>>
  • Breda PC et al. Renal proximal tubular epithelial cells exert immunomodulatory function by driving inflammatory CD4+ T-cell responses. Am J Physiol Renal Physiol. 2019 Apr 24.Read more>>
  • Yu S et al. Dendritic cells modified with Der p1 antigen as a therapeutic potential for allergic rhinitis in a murine model via regulatory effects on IL-4, IL-10 and IL-13.Int Immunopharmacol. 2019 May;70:216-224. Read more>>
  • Shi F et al. Heat injured stromal cells-derived exosomal EGFR enhances prostatic wound healing afterthulium laser resection through EMT and NF-κB signaling. Prostate. 2019 May 24. Read more>>
  • Peruzzaro ST et al. Transplantation of mesenchymal stem cells genetically engineered to overexpress interleukin-10promotes alternative inflammatory response in rat model of traumatic brain injury. J Neuroinflammation. 2019 Jan 5;16(1):2. Read more>>
  • Deng Z et al. M1 macrophage mediated increased reactive oxygen species (ROS) influence wound healing via the MAPK signaling in vitro and in vivo. Toxicol Appl Pharmacol. 2019 Mar 1;366:83-95.Read more>>
  • Jiang L et al. Sonodynamic Therapy in Atherosclerosis by Curcumin Nanosuspensions: Preparation design, Efficacy Evaluation, and Mechanisms Analysis. Eur J Pharm Biopharm. 2020 Jan;146:101-110.Read more>>
  • Liu S et al. Absorbable Thioether Grafted Hyaluronic Acid Nanofibrous Hydrogel for Synergistic Modulation of Inflammation Microenvironment to Accelerate Chronic Diabetic Wound Healing. Adv Healthc Mater. 2020 Apr 27:e2000198.Read more>>
  • Liu S et al. Structural characterization of a novel polysaccharide from Panax notoginseng residue and its immunomodulatory activity on bone marrow dendritic cells. Int J Biol Macromol. 2020 Oct 15;161:797-809.Read more>>
  • Yahima Frin-Herreraet al. Cuban Brown Propolis Interferes in the Crosstalk between Colorectal Cancer Cells and M2 Macrophages. Nutrients. 2020 Jul 9;12(7):2040.Read more>>
  • Yang G et al. A multifunctional anti-inflammatory drug that can specifically target activated macrophages, massively deplete intracellular H2O2, and produce large amounts CO for a highly efficient treatment of osteoarthritis. Biomaterials. 2020 Oct;255:120155.Read more>>
  • Yitian Du. et al. Engineered Microglia Potentiate the Action of Drugs against Glioma Through Extracellular Vesicles and Tunneling Nanotubes. 2021 Feb 28Read more>>
  • Chunai Gong. et al. Regulating the immunosuppressive tumor microenvironment to enhance breast cancer immunotherapy using pH-responsive hybrid membrane-coated nanoparticles. J Nanobiotechnol. 2021 Dec;19(1):1-2Read more>>
  • Tong Shen. et al. Sulfated chitosan rescues dysfunctional macrophages and accelerates wound healing in diabetic mice. Acta Biomater. 2020 Nov;117:192Read more>>
  • Jiyuan Piao. et al. Substance P blocks -aminopropionitrile-induced aortic injury through modulation of M2 monocyte-skewed monocytopoiesis. Transl Res. 2021 Feb;228:76Read more>>
  • Luo Ping. et al. IL-37 inhibits M1-like macrophage activation to ameliorate temporomandibular joint inflammation through the NLRP3 pathway. Rheumatology. 2020 Oct;59(10):3070-308Read more>>
  • Chi Ben. et al. Alternative splicing reverses the cell-intrinsic and cell-extrinsic pro-oncogenic potentials of YAP1. J Biol Chem. 2020 Oct;295:13965Read more>>
  • Hu Lijun. et al. Fusobacterium nucleatum Facilitates M2 Macrophage Polarization and Colorectal Carcinoma Progression by Activating TLR4/NF-B/S100A9 Cascade. Front Immunol. 2021 May;12:1944Read more>>
  • Jie Yu. et al. Synergistic enhancement of immunological responses triggered by hyperthermia sensitive Pt NPs via NIR laser to inhibit cancer relapse and metastasis. Bioact Mater. 2021 MayRead more>>
  • Shuling Zhang. et al. A novel mechanism of lung cancer inhibition by methionine enkephalin through remodeling the immune status of the tumor microenvironment. Int Immunopharmacol. 2021 Oct;99:107999Read more>>
  • Xueli Bai. et al. Regulatory role of methionine enkephalin in myeloid-derived suppressor cells and macrophages in human cutaneous squamous cell carcinoma. Int Immunopharmacol. 2021 Oct;99:107996Read more>>
  • Jie Yu. et al. Combining PD-L1 Inhibitors with Immunogenic Cell Death Triggered by Chemo-Photothermal therapy via a Thermosensitive Liposome System to Stimulate Tumor-specific Immunological Response. Nanoscale. 2021 JulRead more>>
  • Liu, Xiaoqin. et al. Mdivi-1 Modulates Macrophage/Microglial Polarization in Mice with EAE via the Inhibition of the TLR2/4-GSK3-NF-B Inflammatory Signaling Axis. 2021 Oct 07Read more>>
  • Meng Lin. et al. CRISPR-based in situ engineering tumor cells to reprogram macrophages for effective cancer immunotherapy. Nano Today. 2022 Feb;42:101359Read more>>
  • Shanping Wang. et al. Isosteviol Sodium Exerts Anti-Colitic Effects on BALB/c Mice with Dextran Sodium Sulfate-Induced Colitis Through Metabolic Reprogramming and Immune Response Modulation. J Inflamm Res. 2021; 14: 7107C713Read more>>
  • Wang Shanping. et al. Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-_B Pathway. Oxid Med Cell Longev. 2022;2022:4636618Read more>>
  • Singh, Alok Kumar. et al. Re-engineered BCG overexpressing cyclic di-AMP augments trained immunity and exhibits improved efficacy against bladder cancer. Nat Commun. 2022 Feb;13(1):1-16Read more>>
  • Liu, Houli. et al. Immunomodulatory hybrid bio-nanovesicle for self-promoted photodynamic therapy. Nano Res. 2022 Feb;:1-1Read more>>
  • Dai Shi. et al. Synthesis and Evaluation of 68Ga-NOTA-COG1410 Targeting to TREM2 of TAMs as a Specific PET Probe for Digestive Tumor Diagnosis. Anal Chem. 2022;94(9):3819383Read more>>
  • in Wang. et al. Targeted delivery of a STING agonist to brain tumors using bioengineered protein nanoparticles for enhanced immunotherapy. Bioact Mater. 2022 MaRead more>>
  • Mei, Jiawei. et al. An injectable photo-cross-linking silk hydrogel system augments diabetic wound healing in orthopaedic surgery through spatiotemporal immunomodulation. J NANOBIOTECHNOL. 2022 Dec;20(1):1-22Read more>>
  • Kai Ye. et al. An armed oncolytic virus enhances the efficacy of tumor-infiltrating lymphocyte therapy by converting tumors to artificial antigen presenting cells in situ. MOL THER. 2022 JuRead more>>
  • Daijun Zhou. et al. An injectable miR181a-IFI6 nanoparticles promote high-quality healing of radiation-induced skin injury. MATER TODAY ADV. 2022 Aug;15:100267Read more>>
  • Xuan Li. et al. ROS-responsive hydrogel coating modified titanium promotes vascularization and osteointegration of bone defects by orchestrating immunomodulation. BIOMATERIALS. 2022 Aug;287:121683Read more>>
  • Xie, Jian. et al. Aligned electrospun poly(l-lactide) nanofibers facilitate wound healing by inhibiting macrophage M1 polarization via the JAK-STAT and NF-_B pathways. J NANOBIOTECHNOL. 2022 Dec;20(1):1-16Read more>>
  • Xinyan Wu. et al. DNase-1 Treatment Exerts Protective Effects in Neurogenic Pulmonary Edema via Regulating the Neutrophil Extracellular Traps after Subarachnoid Hemorrhage in Mice. J CLIN MED. 2022 Jan;11(15):4349Read more>>
  • Yuqiu Xu. et al. Tumor-infiltrated activated B cells suppress liver metastasis of colorectal cancers. CELL REP. 2022 Aug;40:111295Read more>>
VALIDATION IMAGES

Human U937 Cells were fixed with 2% PFA (10min at room temperature). The cells were then incubated in 1X PBS containing 0.5% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Staining was performed with CD86 Polyclonal Antibody, Unconjugated (bs-1035R) at 1:100 for 30 min on ice. A Goat Anti-rabbit IgG-PE secondary antibody was used for a 30 min incubation on ice. Primary antibody staining (green) is compared to unstained cells (dark blue), secondary antibody only (light blue) and isotype control (bs-0295P; orange).


Mouse Brain cells probed with CD86 Polyclonal Antibody, PE Conjugated (bs-1035R-PE; green) at 1:50 compared to isotype control (bs-0295P-PE; orange).


L1 rat brain lysates L2 rat heart lysates probed with Anti CD86/B7-2 Polyclonal Antibody, Unconjugated (bs-1035R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 34kD. Observed band size:34kD.


Raji cell lysates probed with CD86 Polyclonal Antibody, Unconjugated (bs-1035R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.


Mouse BV-2 Cells were fixed with 4% PFA (10min at room temperature) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. The cells were then incubated in 1X PBS / 2% BSA / 10% goat serum to block non-specific protein-protein interactions for 20 min at 37°C. The cells were stained with CD86 Polyclonal Antibody Unconjugated (bs-1035R; green) at 1:100 for 90 min at 37°C. A FITC-conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) was incubated at 37°C for 90 minutes. DAPI (5ug/ml; blue) was used to stain the cell nuclei.


Lane 1: Human HepG2 cell lysates; Lane 2: Human U937 cell lysates; Lane 3: Rat Spleen lysates; Lane 4: Mouse Spleen lysates; Lane 5: Human HL-60 cell lysates probed with CD86 Polyclonal Antibody, Unconjugated (bs-1035R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


The Daudi(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-CD86 antibody (bs-1035R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-60295G-FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.