Host: Rabbit
Target Protein: HGF
Immunogen Range: 201-300/723
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3082
Swiss Prot: P14210
Source: KLH conjugated synthetic peptide derived from human HGF alpha
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 54/80
Cross Reactive Species:
Human
Mouse
Rat
Sheep
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Yan B et al. Paracrine HGF/c-MET enhances the stem cell-like potential and glycolysis of pancreatic cancer cells via activation of YAP/HIF-1α.Exp Cell Res. 2018 Oct 1;371(1):63-71.Read more>>
- Yu, Xiuju, et al. "Expression and tissue distribution of hepatocyte growth factor (HGF) and its receptor (c-Met) in alpacas (Vicugna pacos) skins associated with white and brown coat colors." Acta Histochemica (2015).Read more>>
- Li, Hongyu, et al. "Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer." Oncology letters9.5 (2015): 1989-1996.Read more>>
- Zhao, Yong, et al. "Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production." Theriogenology (2016).Read more>>
- Wu, Ka, et al. "Biocharacterization of Heat Shock Protein 90 in Acetaminophen-Treated Livers Without Conspicuous Drug Induced Liver Injury." Cellular Physiology and Biochemistry 43.4 (2017): 1-1.Read more>>
- Zhi et al. Effects of PHA-665752 and vemurafenib combination treatment onin vitroand murine xenograft growth of human colorectal cancer cells with BRAFV600Emutations. (2018) Oncol.Lett. 15:3904-3910Read more>>
- Xu X et al. Potential biomarker of fibroblast growth factor 21 in valproic acid-treated livers. Biofactors. 2019 May 23. Read more>>
- Yan B et al. Paracrine HGF/c-MET enhances the stem cell-like potential and glycolysis of pancreatic cancer cells via activation of YAP/HIF-1α.Exp Cell Res. 2018 Oct 1;371(1):63-71.Read more>>
- Quanyu Chen. et al. Hepatocyte growth factor mediates a novel form of hepatic stem/progenitor cell-induced tolerance in a rat xenogeneic liver rejection model. Int Immunopharmacol. 2021 Jan;90:10718Read more>>
- Yan, Liting. et al. HGF can reduce accumulation of inflammation and regulate glucose homeostasis in T2D mice. 2021 Aug 07Read more>>
- Ming Ni. et al. Sox11 Modified Tendon-Derived Stem Cells Promote the Repair of Osteonecrosis of Femoral Head:. Cell Transplant. 2021;()Read more>>
- Liu, Jingyu. et al. Therapeutic effect of adipose stromal vascular fraction spheroids for partial bladder outlet obstruction induced underactive bladder. Stem Cell Res Ther. 2022 Dec;13(1):1-16Read more>>
- Kobayashi, Tsukasa. et al. Prenatal glucocorticoid administration accelerates the maturation of fetal rat hepatocytes. Mol Biol Rep. 2022 Mar;:1-12Read more>>
- Quanyu Chen. et al. Comparative effects of hepatocyte growth factor and tacrolimus on acute liver allograft early tolerance. FRONT IMMUNOL. 2023; 14: 1162439Read more>>
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-HGF Polyclonal Antibody, Unconjugated(bs-1025R) at 1:200, followed by conjugation to the secondary antibody and DAB staining\n
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HGF Polyclonal Antibody, Unconjugated (bs-1025R) at 1:400 overnight at 4°C, DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with HGF polyclonal antibody, unconjugated (bs-1025R) at 1:200 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with HGF polyclonal antibody, unconjugated (bs-1025R) at 1:200 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.