Host: Rabbit
Target Protein: COX4
Immunogen Range: 51-169/169
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1327
Swiss Prot: P13073
Source: KLH conjugated synthetic peptide derived from human COX4
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 17
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
- Wang, Da-Ting, et al. "Artemisinin mimics calorie restriction to trigger mitochondrial biogenesis and compromise telomere shortening in mice." PeerJ 3 (2015): e822.Read more>>
- Wu, Bin, et al. "Succinate-induced neuronal mitochondrial fission and hexokinase II malfunction in ischemic stroke: Therapeutical effects of kaempferol." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2017).Read more>>
- Gao, Jinhang, et al. "De-methylation of displacement loop of mitochondrial DNA is associated with increased mitochondrial copy number and nicotinamide adenine dinucleotide subunit 2 expression in colorectal cancer." Molecular medicine reports 12.5 (2015): 7033-7038.Read more>>
- Kouam et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. (2017) Front.Pharmacol. 8:653Read more>>
- Wang Y et al.Fine particulate matter induces mitochondrial dysfunction and oxidative stress in human SH-SY5Y cells.(2018) Chemosphere;218:577-588. Read more>>
- Zhang JT et al. CRISPR/Cas9 Knockout of Bak Mediates Bax Translocation to Mitochondria in response to TNFα/CHX-induced Apoptosis. BioMed Research International.2019, Article ID 9071297.Read more>>
- Yue Wang # et al. Mitophagy Induced by Mitochondrial Function Damage in Chicken Kidney Exposed to Cr(VI). Biol Trace Elem Res. 2021 Feb;199(2):703-711.Read more>>
- Xudong Xing. et al. Functional annotation map of natural compounds in traditional Chinese medicines library: TCMs with myocardial protection as a case. ACTA PHARM SIN B. 2023 JuRead more>>
Validation submitted by One World Lab. MCF-7 cells were stained with COX4 Polyclonal Antibody, Unconjugated，bs-10257R at 1:50 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
HeLa cells probed with Rabbit Anti-COX4 Polyclonal Antibody (bs-R) at 1:20 for 30 minutes followed by incubation with Goat Anti-Rabbit IgG conjugated secondary FITC (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).\n
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with COX4 Polyclonal Antibody, Unconjugated (bs-10257R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Mouse kidney lysates probed with COX4 Polyclonal Antibody, Unconjugated (bs-10257R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Heart tissue lysates; Lane 3: Mouse Muscle tissue lysates; Lane 4: Rat Kidney tissue lysates; Lane 5: Rat Heart tissue lysates; Lane 6: Rat Muscle tissue lysates; Lane 7: Human HeLa cell lysates; Lane 8: Human Jurkat cell lysates; Lane 9: Human THP-1 cell lysates; Lane 10: Human Raji cell lysates; Lane 11: Human HepG2 cell lysates; Lane 12: Human MCF-7 cell lysates probed with COX4I1 Polyclonal Antibody, Unconjugated (bs-10257R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.