Host: Rabbit
Target Protein: MCT1
Immunogen Range: 251-350/500
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6566
Swiss Prot: P53985
Source: KLH conjugated synthetic peptide derived from human MCT1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 55
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
Dog
Cow
Pig
Horse
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
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Lane 1: A431 lysates; probed with MCT1 Polyclonal Antibody, Unconjugated (bs-10249R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37˚C.
MCF-7 cells probed with MCT1 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated (bs-10249R-A647) at 1:100 for 30 minutes compared to control cells (blue) and isotype control (orange).
K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MCT1 Polyclonal Antibody(bs-10249R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).