bs-10237R [Primary Antibody]
Inhibin Alpha Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Inhibin Alpha

Immunogen Range: 281-366/366


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 16322

Swiss Prot: Q04997

Source: KLH conjugated synthetic peptide derived from mouse Inhibin Alpha

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Inhibin deficient mice are viable but are acutely sensitive to development of gonadal sex-cord stromal tumors.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 15


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded human placenta tissue labeled with Rabbit Anti-Inhibin Alpha Polyclonal Antibody, Unconjugated (bs-10237R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse intestinal lysates probed with Rabbit Anti-Inhibin Alpha Polyclonal Antibody, Unconjugated (bs-10237R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at RT.


Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Inhibin Alpha) Polyclonal Antibody, Unconjugated (bs-10237R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Lane 1: Mouse Ovary tissue lysates; Lane 2: Mouse Placenta tissue lysates; Lane 3: Mouse Testis tissue lysates; Lane 4: Mouse Adrenal gland tissue lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Human K562 cell lysates; Lane 7: Human MCF-7 cell lysates; Lane 8: Human Hela cell lysates; Lane 9: Human SH-SY5Y cell lysates probed with Inhibin Alpha Polyclonal Antibody, Unconjugated (bs-10237R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.