bs-10233R [Primary Antibody]
Nephrin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Nephrin

Immunogen Range: 451-550/1241


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4868

Swiss Prot: O60500

Source: KLH conjugated synthetic peptide derived from human Nephrin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)

Predicted Molecular Weight: 138


Cross Reactive Species: Human

Predicted Cross Reactive Species: Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Fu, Rong, et al. "Podocyte Activation of NLRP3 Inflammasomes Contributes to the Development of Proteinuria in Lupus Nephritis." Arthritis & Rheumatology (2017).Read more>>
  • Chen, Chih-Hung, et al. "Combination therapy of exendin-4 and allogenic adipose-derived mesenchymal stem cell preserved renal function in a chronic kidney disease and sepsis syndrome setting in rats." Oncotarget 8.59 (2017): 100002.Read more>>
  • Maeda et al. CaMK4 compromises podocyte function in autoimmune and nonautoimmune kidney disease. (2018) J.Clin.Invest. 128:3445-3459Read more>>
  • Maeda et al. CaMK4 compromises podocyte function in autoimmune and nonautoimmune kidney disease. (2018) J.Clin.Invest. 128:3445-3459Read more>>
  • Fu R et al. Pim‐1 as a therapeutic target in human lupus nephritis. Arthritis Rheumatol. 2019 Feb 21.Read more>>
  • Xiao-Tan Zhang. et al. Baicalin reversal of DNA hypermethylation-associated Klotho suppression ameliorates renal injury in type 1 diabetic mouse model. Cell Cycle. 2020;19(23):3329-3347Read more>>
  • Fang-Hao Cai. et al. Diagnostic roles of urinary kidney microvesicles in diabetic nephropathy. Ann Transl Med. 2020 Nov; 8(21): 1431Read more>>
  • Hao Li. et al. Interleukin-23 reshapes kidney resident cell metabolism and promotes local kidney inflammation. J Clin Invest. 2021 MayRead more>>
  • Meltem Kuma?-Kulualp. et al. Clemizole hydrochloride, a potent TRPC5 calcium channel inhibitor, prevents cisplatin-induced nephrotoxicity in SpraqueCDawley rats. J BIOCHEM MOL TOXIC. 2023 Apr;:e23372Read more>>
  • Eun-Jeong Kwon. et al. Development of Drug Efficacy Testing Platform for Glomerulonephritis. MICROMACHINES-BASEL. 2024 Mar;15(3):317Read more>>
  • Shin Young Kim. et al. Characterizing Glomerular Barrier Dysfunction with Patient-Derived Serum in Glomerulus-on-a-Chip Models: Unveiling New Insights into Glomerulonephritis. INT J MOL SCI. 2024 Jan;25(10):5121Read more>>
VALIDATION IMAGES

293T cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Nephrin Antibody(bs-10233R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).