bs-1021R-Cy7 [Conjugated Primary Antibody]
TLR4 Polyclonal Antibody, Cy7 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TLR4

Immunogen Range: 751-835/835


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 21898

Swiss Prot: Q9QX05

Source: KLH conjugated synthetic peptide derived from rat TLR4

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion.

Conjugation: Cy7

Excitation/ Emission: 743nm/767nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 90


Cross Reactive Species: Human
Mouse
Rat
Bovine
Cow

Predicted Cross Reactive Species: Dog
Sheep
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Takanashi, Naoya, et al. "Advanced application of bovine intestinal epithelial cell line for evaluating regulatory effect of lactobacilli against heat-killed enterotoxigenic Escherichia coli-mediated inflammation." BMC Microbiology 13.1 (2013): 54.Read more>>
  • Nakayama Y et al. Receptor activator of the NFκB ligand system protects renal function during experimental renal ischemia-reperfusion in mice. Transpl Immunol. 2020 Feb;58:101263.Read more>>
  • Tang, Lu, et al. "Expression of TRAF6 and pro-inflammatory cytokines through activation of TLR2, TLR4, NOD1, and NOD2 in human periodontal ligament fibroblasts." Archives of Oral Biology 56.10 (2011): 1064-1072.Read more>>
  • Chen, Xiaoming, et al. ʺSargassum fusiforme polysaccharide activates nuclear factor kappa-B (NF-κB) and induces cytokine production via Toll-like receptors.ʺ Carbohydrate Polymers (2014).Read more>>
  • Xing, Shanshan, et al. "Relationship between toll-like receptor 4 levels in aorta and severity of atherosclerosis." Journal of International Medical Research (2014): 0300060514534645.Read more>>
  • Wang, Dunjing, et al. "Artesunate Attenuates Lipopolysaccharide-Stimulated Proinflammatory Responses by Suppressing TLR4, MyD88 Expression, and NF-κB Activation in Microglial Cells." Inflammation: 1-8.Read more>>
  • Namisaki, Tadashi, et al. "Beneficial effects of combined ursodeoxycholic acid and angiotensin-II type 1 receptor blocker on hepatic fibrogenesis in a rat model of nonalcoholic steatohepatitis." Journal of Gastroenterology (2015): 1-11.Read more>>
  • ARFIAN, NUR, et al. "Vitamin D Attenuates Kidney Fibrosis via Reducing Fibroblast Expansion, Inflammation, and Epithelial Cell Apoptosis." Kobe J. Med. Sci 62.2 (2016): E38-E44.Read more>>
  • Kawakita, Fumihiro, et al. "Effects of Toll-Like Receptor 4 Antagonists Against Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage in Mice." Molecular Neurobiology (2016): 1-10.Read more>>
  • Harasymowicz, Natalia S., et al. "Regional Differences Between Perisynovial and Infrapatellar Adipose Tissue Depots and Their Response to Class II and III Obesity in Patients with OA." Arthritis & Rheumatology (2017).Read more>>
  • Jenvey, Caitlin J., and Judith R. Stabel. "Sudan Black B masks Mycobacterium avium subspecies paratuberculosis immunofluorescent antibody labeling." Journal of Histology & Histopathology 4.1 (2017): 11.Read more>>
  • Kulhankova, Katarina, et al. "The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function." Infection and immunity (2017): IAI-00848.Read more>>
  • Xu, Wei, et al. "Glaucocalyxin B Alleviates Lipopolysaccharide-Induced Parkinson’s Disease by Inhibiting TLR/NF-κB and Activating Nrf2/HO-1 Pathway." Cellular Physiology and Biochemistry 44.6 (2017): 2091-2104.Read more>>
  • Wang et al. Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin?induced lung inflammation in a murine model of asthma. (2016) Mol.Med.Rep. 13:4289-302Read more>>
  • Qiao et al. Effect of different 1, 25-(OH)2D3 doses on high mobility group box1 and toll-like receptors 4 expression in lung tissue of asthmatic mice. (2015) Int.J.Clin.Exp.Med. 8:4016-23Read more>>
  • Zhao et al. Inhibition of TLR4 Signalling-Induced Inflammation Attenuates Secondary Injury after Diffuse Axonal Injury in Rats. (2016) Mediators.Inflamm. 2016:4706915Read more>>
  • Li C et al. UFL1 Alleviates Lipopolysaccharide-Induced Cell Damage and Inflammation via Regulation of the TLR4/NF-κB Pathway in Bovine Mammary Epithelial Cells. Oxid Med Cell Longev. 2019 Feb 10;2019:6505373. Read more>>
  • Han L et al. Beneficial Effects of Potentilla discolor Bunge Water Extract on Inflammatory Cytokines Release and Gut Microbiota in High-Fat Diet and Streptozotocin-Induced Type 2 Diabetic Mice.Nutrients. 2019 Mar 20;11(3).Read more>>
  • Wang G et al. Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blockingthe TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response. Oxid Med Cell Longev. 2019 Apr 28;2019:7850324.Read more>>
  • Zhang CY et al. Simvastatin alleviates inflammation and oxidative stress in rats with cerebral hemorrhage through Nrf2-ARE signaling pathway. Eur Rev Med Pharmacol Sci. 2019 Jul;23(14):6321-6329. Read more>>
  • Zhan Q et al. Structural characterization and immunomodulatory activity of a novel acid polysaccharide isolated from the pulp of Rosa laevigata Michx fruit. Int J Biol Macromol. 2019 Nov 12. pii: S0141-8130(19)37108-9.Read more>>
  • Li Z et al. Methane-Rich Saline Counteracts Cholestasis-Induced Liver Damage via Regulating the TLR4/NF-κB/NLRP3 Inflammasome Pathway. Oxidative Medicine and Cellular Longevity, 2019, 1–13.Read more>>
  • Winter J et al. Adenine Nucleotide Translocase 1 Expression is Coupled to the HSP27-Mediated TLR4 Signaling in Cardiomyocytes. Cells. 2019 Dec 6;8(12). pii: E1588.Read more>>
  • Nakayama Y et al. Receptor activator of the NFκB ligand system protects renal function during experimental renal ischemia-reperfusion in mice. Transpl Immunol. 2020 Feb;58:101263.Read more>>
  • Peng L et al. Madecassoside Protects Against LPS-Induced Acute Lung Injury via Inhibiting TLR4/NF-B Activation and Blood-Air Barrier Permeability. Front Pharmacol. 2020 Jun 5;11:807.Read more>>
  • Cheng Z et al. Cotinine inhibits TLR4/NF-B signaling pathway and improves deep vein thrombosis in rats. Biosci Rep. 2020 Jun 26;40(6):BSR20201293.Read more>>
  • Chen H et al. Sevoflurane attenuates cognitive dysfunction and NLRP3-dependent caspase-1/11-GSDMD pathway-mediated pyroptosis in the hippocampus via upregulation of SIRT1 in a sepsis model. Arch Physiol Biochem. 2020 Jun 13;1-8.Read more>>
  • Xue Wang. et al. Engineered liposomes targeting the gutCCNS Axis for comprehensive therapy of spinal cord injury. J Control Release. 2021 Mar;331:39Read more>>
  • Xianmei Liu. et al. Tropomodulin1 Expression Increases Upon Maturation in Dendritic Cells and Promotes Their Maturation and Immune Functions. Front Immunol. 2020; 11: 587441Read more>>
  • Shun Huang. et al. Design, Synthesis, and Activity Study of Cinnamic Acid Derivatives as Potent Antineuroinflammatory Agents. Acs Chem Neurosci. 2021;12(3):419C429Read more>>
  • Yuhua Li. et al. Jiaolong capsule protects SD rats against 2,4,6-trinitrobenzene sulfonic acid induced colitis. J Ethnopharmacol. 2021 Apr;269:113716Read more>>
  • Jiajia Wang. et al. TAK-242 ameliorates DSS-induced colitis by regulating the gut microbiota and the JAK2/STAT3 signaling pathway. Microb Cell Fact. 2020 Dec;19(1):1-17Read more>>
  • Lina D. Eissa. et al. Inhibition of thioredoxin-interacting protein and inflammasome assembly using verapamil mitigates diabetic retinopathy and pancreatic injury. Eur J Pharmacol. 2021 Mar;:174061Read more>>
  • Lili Li. et al. Protein overexpression of toll?like receptor 4 and myeloid differentiation factor 88 in oral squamous cell carcinoma and clinical significance. Oncol Lett. 2021 Nov;22(5):1-11Read more>>
  • Sun, Lizhong. et al. Fibroblast membrane-camouflaged nanoparticles for inflammation treatment in the early stage. Int J Oral Sci. 2021 Nov;13(1):1-14Read more>>
  • Aykut Ozturk. et al. The protective effects of trimetazidine against ovary ischemiareperfusion injury via the TLR4/Nf-kB signal pathway. J BIOCHEM MOL TOXIC. 2022 May 27Read more>>
  • Mona F. El-Azab. et al. A novel role of Nano selenium and sildenafil on streptozotocin-induced diabetic nephropathy in rats by modulation of inflammatory, oxidative, and apoptotic pathways. LIFE SCI. 2022 Aug;303:120691Read more>>
  • Chunyan Lei. et al. HMGB1/TLR4 induces autophagy and promotes neuroinflammation after intracerebral hemorrhage. BRAIN RES. 2022 Jul;:148003Read more>>
  • Irene Palenca. et al. N-Palmitoyl-D-Glucosamine Inhibits TLR-4/NLRP3 and Improves DNBS-Induced Colon Inflammation through a PPAR-α-Dependent Mechanism. BIOMOLECULES. 2022 Aug;12(8):1163Read more>>
  • Canan Ero?lu Gne?. et al. Glycoside oleandrin downregulates toll-like receptor pathway genes and associated miRNAs in human melanoma cells. GENE. 2022 Aug;:146805Read more>>
  • Ruiyue Sun. et al. Effects of Bacillus subtilis natto JLCC513 on gut microbiota and intestinal barrier function in obese rats. J APPL MICROBIOL. 2022 SepRead more>>
  • Jingwen Liu. et al. The effects of Saccharomyces boulardii on rat colonic hypermotility induced by repeated water avoidance stress and the potential mechanism. PEERJ. 2022 Nov;10:e1439Read more>>
  • aoming Tian. et al. Modulating effects of Hericium erinaceus polysaccharides on the immune response by regulating gut microbiota in cyclophosphamide-treated mice. J SCI FOOD AGR. 2022 DecRead more>>
  • Jianhao Yang. et al. Serum amyloid A regulates TLR2/4-mediated IFN- signaling pathway against Marek's disease virus. VIRUS RES. 2023 Jan;:199044Read more>>
  • Siyu Qiang. et al. Changes in the content of Puerarin-PLGA nanoparticles in mice under the influence of alcohol and analysis of their antialcoholism. J APPL BIOMATER FUNC. ;()Read more>>
  • aoming Tian. et al. Ameliorating effects of Hericium erinaceus polysaccharides on intestinal barrier injury in immunocompromised mice induced by cyclophosphamide. FOOD FUNCT. 2023 FebRead more>>
  • Z. Chen. et al. Mechanism of Resveratrol Improving Rheumatoid Arthritis Injury by Inhibiting S100A8/A9 Expression. INDIAN J PHARM SCI. 2023 Feb;85(1):145-151Read more>>
  • Yifan Zhu. et al. Discovery of Selective P2Y6R Antagonists with High Affinity and In Vivo Efficacy for Inflammatory Disease Therapy. J MED CHEM. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Xiahong Tang. et al. Hypoxic preconditioned mesenchymal stem cells ameliorate rat brain injury after cardiopulmonary resuscitation by suppressing neuronal pyroptosis. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 MayRead more>>
  • Yixuan Liang. et al. Dihydrotestosterone mediates the inflammation effect under lipopolysaccharides in bovine endometrial epithelial cells via AR blockading TLR4/MyD88 signaling pathway. ANIM REPROD SCI. 2023 Aug;255:107292Read more>>
VALIDATION IMAGES