bs-10216R
[Primary Antibody]
SOD1 Polyclonal antibody
DATASHEET
Host: Rabbit
Target Protein: SOD1
Immunogen Range: 6-100/154
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6647
Source: KLH conjugated synthetic peptide derived from human SOD1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 17
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Cow
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wang Z et al. Aronia melanocarpa Prevents Alcohol-Induced Chronic Liver Injury via Regulation of Nrf2 Signaling in C57BL/6 Mice. Oxid Med Cell Longev. 2020 Jan 8;2020:4054520.Read more>>
- Xu et al. Morphological and proteomic analyses reveal that unsaturated guluronate oligosaccharide modulates multiple functional pathways in murine macrophage RAW264.7 cells. (2015) Mar.Drug. 13:1798-818Read more>>
- Han Y et al. Inonotus obliquus polysaccharides protect against Alzheimer's disease by regulating Nrf2 signaling and exerting antioxidative and antiapoptotic effects.Int J Biol Macromol. 2019 Jun 15;131:769-778. Read more>>
- Meng B et al. Hepatoprotective Effects of Morchella esculenta against Alcohol-Induced Acute Liver Injury in the C57BL/6 Mouse Related to Nrf-2 and NF-κB Signaling. Oxidative Medicine and Cellular Longevity.2019, Article ID 6029876.Read more>>
- Arfian N et al. Chlorogenic Acid Attenuates Kidney Ischemic/Reperfusion Injury via Reducing Inflammation, Tubular Injury, and Myofibroblast Formation. BioMed Research International, 2019, 1–10. Read more>>
- Wang Z et al. Aronia melanocarpa Prevents Alcohol-Induced Chronic Liver Injury via Regulation of Nrf2 Signaling in C57BL/6 Mice. Oxid Med Cell Longev. 2020 Jan 8;2020:4054520.Read more>>
- Liu R et al. Effect of Dietary Methionine Deficiency Followed by a Re-Feeding Phase on the Hepatic Antioxidant Activities of LambsAnimals (Basel).2020 Dec 23;11(1):7.Read more>>
- Yukun Fang. et al. Activation of the ROS/HO-1/NQO1 signaling pathway contributes to the copper-induced oxidative stress and autophagy in duck renal tubular epithelial cells. Sci Total Environ. 2021 Feb;757:143753Read more>>
- Jiyoung Hwang. et al. SOD1 suppresses pro-inflammatory immune responses by protecting against oxidative stress in colitis. Redox Biol. 2020 Oct;37:10176Read more>>
- Shaofeng Wu. et al. Protective effects of curcumin on ATO-induced nephrotoxicity in ducks in relation to suppressed autophagy, apoptosis and dyslipidemia by regulating oxidative stress. Ecotox Environ Safe. 2021 Aug;219:11235Read more>>
- Xinrui Zhang. et al. Wan-Nian-Qing, a Herbal Composite Prescription, Suppresses the Progression of Liver Cancer in Mice by Regulating Immune Response. Front Oncol. 2021; 11: 696282Read more>>
- Setyaningsih Wiwit A. W.. et al. Ethanolic Extract of Centella asiatica Treatment in the Early Stage of Hyperglycemia Condition Inhibits Glomerular Injury and Vascular Remodeling in Diabetic Rat Model. Evid-Based Compl Alt. 2021;2021:667113Read more>>
- Zhang-Jie Yang. et al. SIRT 3 was involved in Lycium barbarum seed oil protection testis from oxidative stress: in?vitro and in?vivo analyses. Pharm Biol. 2021;59(1):1314-1325Read more>>
- Shaofeng Wu. et al. The neuroprotective effect of curcumin against ATO triggered neurotoxicity through Nrf2 and NF-B signaling pathway in the brain of ducks. Ecotox Environ Safe. 2021 Dec;228:112965Read more>>
- Yongfeng Zhang. et al. Aucubin slows the development of osteoporosis by inhibiting osteoclast differentiation via the nuclear factor erythroid 2-related factor 2-mediated antioxidation pathway. Pharm Biol. 2021;59(1):1556-1565Read more>>
- Yue Song. et al. Maternal ochratoxin A exposure impairs meiosis progression and primordial follicle formation of F1 offspring. FOOD CHEM TOXICOL. 2022 Aug;:113386Read more>>
- Ai-Wen Kang. et al. Puerarin extends the lifespan of Drosophila melanogaster by activating autophagy. FOOD FUNCT. 2023 FebRead more>>
- Yanan Hao. et al. Alginate Oligosaccharides Repair Liver Injury by Improving Anti-Inflammatory Capacity in a Busulfan-Induced Mouse Model. INT J MOL SCI. 2023 Jan;24(4):3097Read more>>
- Ting Yao. et al. Differences of the anti-oxidative capability, GPX3, and Cu/ZnSOD expression in Hu sheep testis with different size at six-month-old. ANIM BIOTECHNOL. 2023 Feb 15Read more>>
- Huihui Sun. et al. Study of anti-fatigue activity of polysaccharide from fruiting bodies of Armillaria gallica. INT J BIOL MACROMOL. 2023 Apr;:124611Read more>>
- Depeng Fang. et al. ROS-responsive nanocomposite scaffolds for sustained releasing puerarin to achieve chondroprotection in OA rats. MATER DESIGN. 2023 Aug;:112214Read more>>
- Gan Rao. et al. Arsenic and polystyrene-nano plastics co-exposure induced testicular toxicity: Triggers oxidative stress and promotes apoptosis and inflammation in mice. ENVIRON TOXICOL. 2023 SepRead more>>
- Hongwei Duan. et al. The mechanism of curcumin to protect mouse ovaries from oxidative damage by regulating AMPK/mTOR mediated autophagy. PHYTOMEDICINE. 2024 Feb;:155468Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-SOD1 Polyclonal Antibody, Unconjugated (bs-10216R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-SOD1 Polyclonal Antibody, Unconjugated (bs-10216R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Mouse brain lysates probed with Rabbit Anti-SOD1 Polyclonal Antibody, Unconjugated (bs-10216R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT.
Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with SOD1 Polyclonal antibody, Unconjugated (bs-10216R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SOD1 Polyclonal Antibody, Unconjugated (bs-10216R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SOD1 Polyclonal Antibody, Unconjugated (bs-10216R) at 1:200 overnight at 4°C, DAB staining.
Jurkat cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SOD1 Polyclonal Antibody(bs-10216R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).