bs-1020R-Cy5 [Conjugated Primary Antibody]
ERK1 Polyclonal Antibody, Cy5 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ERK1

Specificity: Possible cross-reactivity with ERK2

Immunogen Range: 101-200/380


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 26417

Swiss Prot: Q63844

Source: KLH conjugated synthetic peptide derived from human ERK1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1. Phosphorylates heat shock factor protein 4(HSF4) (By similarity). Highest levels within the nervous system, expressed in different tissues, mostly in intestine, placenta and lung. Increased expression during development. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain. Binds both upstream activators and downstream substrates in multimolecular complexes. Found in a complex with at least BRAF, HRAS, MAP2K1/MEK1, MAPK3 and RGS14. Interacts with TPRBy similarity. Interacts with ADAM15, ARRB2, CANX, DAPK1 (via death domain), HSF4, IER3, MAP2K1/MEK1, NISCH, and SGK1By similarity. Interacts with MORG1, PEA15 and MKNK2. MKNK2 isoform 1 binding prevents from dephosphorylation and inactivation.

Conjugation: Cy5

Excitation/ Emission: 625,650nm/670nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 43


Cross Reactive Species: Human
Mouse
Rat
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li, Lingrui, et al. "Nrf2/ARE pathway activation, HO-1 and NQO1 induction by polychlorinated biphenyl quinone is associated with reactive oxygen species and PI3K/AKT signaling." Chemico-Biological Interactions (2013).Read more>>
  • Oyagbemi AA et al. Quercetin attenuates hypertension induced by sodium fluoride via reduction in oxidative stress and modulation of HSP 70/ERK/PPARγ signaling pathways. (2018) Sep 03Read more>>
  • Chi Q et al. Hydrogen Sulfide Gas Exposure Induces Necroptosis and Promotes Inflammation through the MAPK/NF-κB Pathway in Broiler Spleen. Oxid Med Cell Longev. 2019 Jul 31;2019:8061823. Read more>>
  • Hongyi Qu. et al. The effects of vasoactive intestinal peptide on RANKL-induced osteoclast formation. Ann Transl Med. 2021 Jan; 9(2): 127Read more>>
  • Chaoqun Wang. et al. Cl-amidine attenuates lipopolysaccharide-induced mouse mastitis by inhibiting NF-B, MAPK, NLRP3 signaling pathway and neutrophils extracellular traps release. Microb Pathogenesis. 2020 Dec;149:10453Read more>>
  • Yuqiu Xu. et al. Tumor-infiltrated activated B cells suppress liver metastasis of colorectal cancers. CELL REP. 2022 Aug;40:111295Read more>>
  • Huawei Liu. et al. Integrated multi-omics reveals the beneficial role of chlorogenic acid in improving the growth performance and immune function of immunologically-stressed broilers. ANIM NUTR. 2023 MayRead more>>
VALIDATION IMAGES