Host: Rabbit
Target Protein: ALDH1
Immunogen Range: 201-300/501
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 216
Swiss Prot: P00352
Source: KLH conjugated synthetic peptide derived from human ALDH1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 54
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Cow
Sheep
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Zhou, Jiahui, et al. "microRNA-143 is associated with the survival of ALDH1+ CD133+ osteosarcoma cells and the chemoresistance of osteosarcoma."Experimental Biology and Medicine (2015).Read more>>
- Tang, Xin, et al. "Downregulation of CXCR7 inhibits proliferative capacity and stem cell-like properties in breast cancer stem cells." Tumor Biology (2016): 1-9.Read more>>
- Luo, Chengxin, et al. "Upregulation of the transient receptor potential vanilloid 1 in colonic epithelium of patients with active inflammatory bowel disease." Int J Clin Exp Pathol 10.11 (2017): 11335-11344.Read more>>
- Qi et al. Sorting and identification of side population cells in the human cervical cancer cell line HeLa. (2014) Cancer.Cell.Int. 14:3Read more>>
- Wang L et al. Lung CSC\derived exosomal miR\210\3p contributes to a pro\metastatic phenotype in lung cancer by targeting FGFRL1. J Cell Mol Med. 2020 Jun;24(11):6324-6339.Read more>>
- Yohei Sekino. et al. BUB1B Overexpression Is an Independent Prognostic Marker and Associated with CD44, p53, and PD-L1 in Renal Cell Carcinoma. Oncology-Basel. 2021;99(4):240-25Read more>>
- Chih-Wei Chou. et al. Menin and Menin-Associated Proteins Coregulate Cancer Energy Metabolism. Cancers. 2020 Sep;12(9):2715Read more>>
- Sijie Wang. et al. PFKFB4 facilitates palbociclib resistance in oestrogen receptor-positive breast cancer by enhancing stemness. CELL PROLIFERAT. 2022 Sep;:e13337Read more>>
- Mingqian Fang. et al. Lipopolysaccharide-binding protein expression is increased by stress and inhibits monoamine synthesis to promote depressive symptoms. IMMUNITY. 2023 Feb 27Read more>>
- Xixi Fei. et al. Molecular characterization and expression profile of the ALDH1A1 gene and its functions in yak luteal cells. THERIOGENOLOGY. 2024 Jul;223:98Read more>>
- Jinmiao Tian. et al. QPH-FR: A Novel Quinoa Peptide Enhances Chemosensitivity by Targeting Leucine-Rich Repeat-Containing G Protein-Coupled Receptor 5 in Colorectal Cancer. J AGR FOOD CHEM. 2024;XXXX(XXX):XXX-XXXRead more>>
Image obtained from One World Lab validations program. A549 cells were stained with ALDH1 Polyclonal Antibody, Unconjugated (bs-10162R) at 1:50 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-ALDH1 Polyclonal Antibody, Unconjugated (bs-10162R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded human liver carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-ALDH1 Polyclonal Antibody, Unconjugated (bs-10162R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Human A549 and HepG2 cells probed with ALDH1 Polyclonal Antibody, unconjugated (bs-10162R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:20000 for 60 minutes at 37°C.