bs-10138R [Primary Antibody]
Eph receptor B1 (Tyr928) Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Eph receptor B1 Tyr928

Modification Site: Tyr928

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2047

Swiss Prot: P54762

Source: KLH conjugated synthetic phosphopeptide derived from human EphB1 around the phosphorylation site of Tyr928

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 106


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti phospho-EphB1/Eph receptor B1(Tyr928) Antibody, Unconjugated (bs-10138R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse ovary lysates probed with Eph receptor B1 (Tyr928) Polyclonal Antibody, Unconjugated (bs-10138R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human U87MG cell lysates; Lane 5: Human MCF-7 cell lysates; Lane 6: Human U-2 OS cell lysates probed with phospho-Eph receptor B1 (Tyr928) Polyclonal Antibody, Unconjugated (bs-10138R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.