bs-10133R [Primary Antibody]
AKT1/2/3 (Tyr474) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: AKT1/2/3 Tyr474

Specificity: This antibody detects Akt1 when phosphorylated at Tyr474 and correspondingly, Akt2 when phosphorylated at Tyr 475 and Akt3 when phosphorylated at Tyr473

Modification Site: Tyr474

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 207

Source: KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Tyr474

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 56


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Pig
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xinyang Fan. et al. CEBPA-Regulated Expression of SOCS1 Suppresses Milk Protein Synthesis through mTOR and JAK2-STAT5 Signaling Pathways in Buffalo Mammary Epithelial Cells. FOODS. 2023 Jan;12(4):708Read more>>
  • Xinyang Fan. et al. MiR-190a regulates milk protein biosynthesis through the mTOR and JAK2CSTAT5 signaling pathways by targeting PTHLH in buffalo mammary epithelial cells. J FUNCT FOODS. 2023 Mar;102:105451Read more>>
  • Xianrong Xiong. et al. Maternal Kdm2a-mediated PI3K/Akt signaling and E-cadherin stimulate the morula-to-blastocyst transition revealing crucial roles in early embryonic development. THERIOGENOLOGY. 2023 Oct;209:6Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human myometrium labeled with Anti-phospho-AKT1 (Tyr474)Polyclonal Antibody, Unconjugated (bs-10133R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse kidney lysates probed with AKT1/2/3 (Tyr474) Polyclonal Antibody, unconjugated (bs-10133R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.


Mouse lung lysates probed with AKT1/2/3 (Tyr474) Polyclonal Antibody, unconjugated (bs-10133R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.