bs-10125R [Primary Antibody]
SLC7A5 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SLC7A5

Immunogen Range: 101-200/507


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8140

Swiss Prot: Q01650

Source: KLH conjugated synthetic peptide derived from human SLC7A5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 55


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Beals, Joseph W., et al. "Anabolic sensitivity of postprandial muscle protein synthesis to the ingestion of a protein-dense food is reduced in overweight and obese young adults." The American Journal of Clinical Nutrition (2016): ajcn130385.Read more>>
  • Mayers, Jared R., et al. "Tissue of origin dictates branched-chain amino acid metabolism in mutant Kras-driven cancers." Science 353.6304 (2016): 1161-1165.Read more>>
  • Reyer, Henry, et al. "Possible molecular mechanisms by which an essential oil blend from star anise, rosemary, thyme, and oregano and saponins increase the performance and ileal protein digestibility of growing broilers." Journal of Agricultural and Food Chemistry (2017).Read more>>
  • van Vliet S et al. Time-dependent regulation of postprandial muscle protein synthesis rates after milk protein ingestion in young men. J Appl Physiol (1985). 2019 Nov 14. Read more>>
  • C. Goldeman. et al. Human induced pluripotent stem cells (BIONi010-C) generate tight cell monolayers with blood-brain barrier traits and functional expression of large neutral amino acid transporter 1 (SLC7A5). Eur J Pharm Sci. 2021 Jan;156:105577Read more>>
  • Haochen Guo. et al. Polymeric ligands comprising sulfur-containing amino acids for targeting tumor-associated amino acid transporters. BIOMATERIALS. 2023 Feb;293:121987Read more>>
  • Sun-Yee Kim. et al. Genetic Ablation of LAT1 Inhibits Growth of Liver Cancer Cells and Downregulates mTORC1 Signaling. INT J MOL SCI. 2023 Jan;24(11):9171Read more>>
VALIDATION IMAGES

Mouse brain lysates probed with Rabbit Anti-SLC7A5 Polyclonal Antibody (bs-10125R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 55kDa