bs-1011R [Primary Antibody]
CXCR4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CXCR4

Immunogen Range: 201-294/352


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7852

Source: KLH conjugated synthetic peptide derived from human CXCR4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 40


Cross Reactive Species: Human
Mouse
Rat
Rabbit

Predicted Cross Reactive Species: Cow

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Pei, Guangchang, et al. "Renal Interstitial Infiltration and Tertiary Lymphoid Organ Neogenesis in IgA Nephropathy." Clinical Journal of the American Society of Nephrology (2013): CJN-01150113.Read more>>
  • Zhuo, Wei, et al. ʺThe CXCL12–CXCR4 Chemokine Pathway: A Novel Axis Regulates Lymphangiogenesis.ʺ Clinical Cancer Research 18.19 (2012): 5387-5398.Read more>>
  • Lu Z, Qi L, Bo XJ, Liu GD, Wang JM, Li G. Expression of CD26 and CXCR4 in prostate carcinoma and its relationship with clinical parameters. CD26 and CXCR4 expression shows correlation with prostate cancer. J Res Med Sci 2013;18:647-52Read more>>
  • Wu, Qiang, et al. "B-cell lymphoma 6 protein stimulates oncogenicity of human breast cancer cells." BMC Cancer 14.1 (2014): 418.Read more>>
  • Li, Yanlei, et al. "Co-expression of uPAR and CXCR4 promotes tumor growth and metastasis in small cell lung cancer." International Journal of Clinical Experimental Pathology 7.7 (2014): 3771-3780.Read more>>
  • Zhou, Hao, et al. "Exendin-4 enhances the migration of adipose-derived stem cells to neonatal rat ventricular cardiomyocyte-derived conditioned medium via the phosphoinositide 3-kinase/Akt-stromal cell-derived factor-1α/CXC chemokine receptor 4 pathway." Molecular Medicine Reports.Read more>>
  • Mori, Miki, et al. "Stromal Cell-Derived Factor-1α Plays a Crucial Role Based on Neuroprotective Role in Neonatal Brain Injury in Rats." International Journal of Molecular Sciences 16.8 (2015): 18018-18032.Read more>>
  • Zhou, Hao, et al. "Effects of Exendin-4 on bone marrow mesenchymal stem cell proliferation, migration and apoptosis in vitro." Scientific Reports 5 (2015).Read more>>
  • Mu, Hailong, et al. "PLZF‐Induced Upregulation of CXCR4 Promotes Dairy Goat Male Germline Stem Cell Proliferation by Targeting Mir146a." Journal of Cellular Biochemistry (2015).Read more>>
  • Wang, Xiao-yan, et al. "AMD3100 attenuates MMP-3 and MMP-9 expressions and prevents cartilage degradation in a monosodium iodoacetate-induced rat model of temporomandibular osteoarthritis." Journal of Oral and Maxillofacial Surgery (2016).Read more>>
  • Aerbajinai, Wulin, et al. "Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin." Journal of Biological Chemistry291.16 (2016): 8549-8564.Read more>>
  • Ochi, Akinobu, et al. "MIF-2/D-DT Enhances Proximal Tubular Cell Regeneration Through SLPI and ATF4-dependent Mechanisms." American Journal of Physiology-Renal Physiology (2017).Read more>>
  • Fontana et al. Impact of Steroids on the Inflammatory Response after Ischemic Acute Kidney Injury in Rats. (2017) Indian.J.Nephro. 27:365-371Read more>>
  • Liu et al. CXCR4-overexpressing bone marrow-derived mesenchymal stem cells improve repair of acute kidney injury. (2013) Am.J.Physiol.Renal.Physio. 305:F1064-73Read more>>
  • Zhou,et al.CXCR4 antagonist AMD3100 enhances the response of MDA-MB-231 triple-negative breast cancer cells to ionizing radiation.(2018) Cancer Letters. 418:196-203.Read more>>
  • Jolly,et al.Targeted endothelial gene deletion of Triggering Receptor Expressed on Myeloid cells-1 protects mice during septic shock.(2018) Cardiovascular Research. 114:907-918.Read more>>
  • Colas,et al.Impaired Production and Diurnal Regulation of Vascular RvDn-3 DPA Increase Systemic Inflammation and Cardiovascular Disease.(2018) Circulation Research. 122:855-863.Read more>>
  • Expression of Pref-1 and Related Chemokines during theDevelopment of Rat Mesenteric Lymph Nodes.(2018)Biomed Environ Sci.ul;31(7):507-514. Read more>>
  • Sha Y et al. MGF E peptide improves anterior cruciate ligament repair by inhibiting hypoxia‐induced cell apoptosis and accelerating angiogenesis.(2018) J Cell Physiol. 2018 Oct 14. Read more>>
  • Zhuang M et al. Reelin regulates male mouse reproductive capacity via the sertoli cells.(2018) J. Cell. Biochem. 2018 Oct 18Read more>>
  • Sheu JJ et al. Therapeutic effects of adipose derived fresh stromal vascular fraction-containing stem cellsversus cultured adipose derived mesenchymal stem cells on rescuing heart function in rat after acute myocardial infarction. Am J Transl Res. 2019 Jan 15;11(1):67-86. eCollection 2019.Read more>>
  • Yin TC et al. Extracorporeal shock wave-assisted adipose-derived fresh stromal vascular fraction restores the blood flow of critical limb ischemia in rat. Vascul Pharmacol. 2019 Feb;113:57-69. Read more>>
  • Toyoma S et al. SDF-1/CXCR4 induces cell invasion through CD147 in squamous cell carcinoma of the hypopharynx. Oncol Lett. 2020 Aug;20(2):1817-1823.Read more>>
  • Sabrina Spiller. et al. Protease-Triggered Release of Stabilized CXCL12 from Coated Scaffolds in an Ex Vivo Wound Model. Pharmaceutics. 2021 Oct;13(10):1597Read more>>
  • Qun Lin. et al. Silencing CTNND1 Mediates Triple-Negative Breast Cancer Bone Metastasis via Upregulating CXCR4/CXCL12 Axis and Neutrophils Infiltration in Bone. Cancers. 2021 Jan;13(22):5703Read more>>
  • Yanxin Li. et al. Decoding the temporal and regional specification of microglia in the developing human brain. Cell Stem Cell. 2022 MaRead more>>
  • Kawabata, Shuhei. et al. Association of Gut Microbiome with Early Brain Injury After Subarachnoid Hemorrhage: an Experimental Study. TRANSL STROKE RES. 2022 Dec;:1-14Read more>>
VALIDATION IMAGES

Human U937 cells probed withCXCR4 Polyclonal Antibody, Unconjugated (bs-1011R) at 1:100 for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


L1 rat brain lysates L2 rat kidney lysates probed with Anti- CD184/CXCR4 Polyclonal Antibody, Unconjugated (bs-1011R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 39kD.Observed band 39kD


Mouse lung lysates probed with Anti-CXCR4 Polyclonal Antibody (bs-1011R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.


Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD184/CXCR4 Polyclonal Antibody (bs-1011R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-CD184/CXCR4 Polyclonal Antibody (bs-1011R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (bs-1011R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (bs-1011R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (bs-1011R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human prostate tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (bs-1011R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (bs-1011R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human esophageal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (bs-1011R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.