bs-1009R [Primary Antibody]
CXCR1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CXCR1

Immunogen Range: 186-280/350


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3577

Swiss Prot: P25024

Source: KLH conjugated synthetic peptide derived from human CXCR1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 40


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Cow
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Tang, Cui, et al. "High expression of GPER1, EGFR and CXCR1 is associated with lymph node metastasis in papillary thyroid carcinoma." International Journal of Clinical and Experimental Pathology 7.6 (2014): 3213-3223.Read more>>
  • Schneberger, D., et al. "CXCR1/CXCR2 antagonist CXCL8 (3-74) K11R/G31P blocks lung inflammation in swine barn dust-instilled mice." Pulmonary Pharmacology & Therapeutics (2015).Read more>>
  • Zhao, Le, et al. "Role of GPER1, EGFR and CXCR1 in differentiating between malignant follicular thyroid carcinoma and benign follicular thyroid adenoma."International Journal of Clinical and Experimental Pathology 8.9 (2015): 11236-11247.Read more>>
  • Tang et al. High expression of GPER1, EGFR and CXCR1 is associated with lymph node metastasis in papillary thyroid carcinoma. (2014) Int.J.Clin.Exp.Patho. 7:3213-23Read more>>
  • Guangxu Xiao. et al. CXCR1 and its downstream NF-B inflammation signaling pathway as a key target of Guanxinning injection for myocardial ischemia/reperfusion injury.. FRONT IMMUNOL. 2022 Oct;13:1007341-1007341Read more>>
  • Chengfei Xu. et al. CXCR1 participates in bone cancer pain induced by Walker 256 breast cancer cells in female rats. MOL PAIN. ;()Read more>>
  • Yuan Li. et al. A Biomimetic Peptide Functions as Specific Extracellular Matrix for Quiescence of Stem Cells against Intervertebral Disc Degeneration. SMALL. 2023 Jul;:2300578Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat colitis tissue labeled with Anti-CXCR1/IL-8RA Polyclonal Antibody, Unconjugated (bs-1009R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Lane 1: rat kidney lysates Lane 2: rat brain lysates probed with Anti CXCR1/IL-8RA Polyclonal Antibody, Unconjugated (bs-1009R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 40kD. Observed band size: 40kD.


Lane 1: Human Jurkat cell Lysates; Lane 2: Human Raji cell Lysates. Probed with CXCR1 polyclonal Antibody, unconjugated (bs-1009R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Lung tissue lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human Raji cell lysates probed with CXCR1 Polyclonal Antibody, Unconjugated (bs-1009R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Lymph node tissue lysates; Lane 2: Rat Lymph node cell lysates probed with CXCR1 Polyclonal Antibody, Unconjugated (bs-1009R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.