bs-1004R
[Primary Antibody]
ACE2 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: ACE2
Immunogen Range: 601-700/805
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 59272
Swiss Prot: Q9BYF1
Source: KLH conjugated synthetic peptide derived from human ACE2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 87
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Sheep
Pig
Bat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Raffai, Gabor, Gilson Khang, and Paul M. Vanhoutte. "Angiotensin-(1-7) augments endothelium-dependent relaxations of porcine coronary arteries to bradykinin by inhibiting ACE1." Journal of Cardiovascular Pharmacology (2014).Read more>>
- Guo, Jun, and Changting Liu. "Protective Effects of Telmisartan in a Rat Model of Pulmonary Arterial Hypertension." Experimental & Clinical Cardiology (2014).Read more>>
- Liu H et al. Ginsenoside Rg3 Attenuates Angiotensin II-Mediated Renal Injury in Rats and Mice by Upregulating Angiotensin-Converting Enzyme 2 in the Renal Tissue. Evid Based Complement Alternat Med. 2019 Nov 29;2019:6741057.Read more>>
- Wu C et al. Potential Simultaneous Inhibitors of Angiotensin-Converting Enzyme 2 and Transmembrane Protease, Serine 2Front Pharmacol.2020 Dec 17;11:584158.Read more>>
- Wu Bingshan. et al. Single-Cell Sequencing of Glioblastoma Reveals Central Nervous System Susceptibility to SARS-CoV-2. Front Oncol. 2020 Nov;10:2534Read more>>
- Ching-Yuan Wu. et al. GB-2 inhibits ACE2 and TMPRSS2 expression: In vivo and in vitro studies. Biomed Pharmacother. 2020 Dec;132:110816Read more>>
- Giorgio A. Croci. et al. Emergency Lung Transplantation after COVID-19: Immunopathological Insights on Two Affected Patients. Cells-Basel. 2021 Mar;10(3):611Read more>>
- Luca Elli. et al. ANTI-SARS-CoV-2 IMMUNOGLOBULIN PROFILE IN PATIENTS WITH CELIAC DISEASE LIVING IN A HIGH INCIDENCE AREA. Digest Liver Dis. 2021 SepRead more>>
- Wu, Meng-Li. et al. SARS-CoV-2-triggered mast cell rapid degranulation induces alveolar epithelial inflammation and lung injury. Signal Transduct Tar. 2021 Dec;6(1):1-15Read more>>
- Dolaji Henin. et al. Morphological and Immunopathological Aspects of Lingual Tissues in COVID-19. CELLS-BASEL. Cells-Basel. 2022 Jan;11(7):1248Read more>>
- Virginia Lotti. et al. CFTR Modulation Reduces SARS-CoV-2 Infection in Human Bronchial Epithelial Cells. CELLS-BASEL. 2022 Jan;11(8):1347Read more>>
- Liang-Hsuan Chien. et al. Study on the potential of Sanghuangporus sanghuang and its components as COVID-19 spike protein receptor binding domain inhibitors. BIOMED PHARMACOTHER. 2022 Sep;153:113434Read more>>
- Mercado-Gmez, Maria. et al. The spike of SARS-CoV-2 promotes metabolic rewiring in hepatocytes. COMMUN BIOL. 2022 Aug;5(1):1-13Read more>>
- Jing-Chao Su. et al. The Pretreatment of Xiaoqinglong Decoction Alleviates Inflammation and Oxidative Damage and Up-Regulates Angiotensin-Converting Enzyme 2 in Lipopolysaccharide-Induced Septic Acute Lung Injury Rats. EVID-BASED COMPL ALT. 2022 Sep 19;2022:2421198Read more>>
- Haoyu Sun. et al. Increased circulating microparticles contribute to severe infection and adverse outcomes of COVID-19 patients with diabetes. AM J PHYSIOL-HEART C. 2022 Oct;(Articles in Press)Read more>>
- Te-Kai Sun. et al. Schizophyllum commune Reduces Expression of the SARS-CoV-2 Receptors ACE2 and TMPRSS2. INT J MOL SCI. 2022 Jan;23(23):14766Read more>>
- Michinori Ishitoku. et al. Tumor Necrosis Factor and Interleukin-1 Upregulate NRP2 Expression and Promote SARS-CoV-2 Proliferation. VIRUSES-BASEL. 2023 Jul;15(7):1498Read more>>
- Ching-Yuan Wu. et al. The anti-SARS-CoV-2 effect and mechanism of Chiehyuan herbal oral protection solution. HELIYON. 2023 Jul;9:e17701Read more>>
- Wen-Ping Jiang. et al. Anti-SARS-CoV-2 Viral Activity of Sweet Potato Trypsin Inhibitor via Downregulation of TMPRSS2 Activity and ACE2 Expression In Vitro and In Vivo. INT J MOL SCI. 2024 Jan;25(11):6067Read more>>
- Angus K Jacobs. et al. Hepatic angiotensin-converting enzyme 2 expression in metabolic dysfunction-associated steatotic liver disease and in patients with fatal COVID-19. WORLD J GASTROENTERO. 2024 Aug 21; 30(31): 37053716Read more>>
VALIDATION IMAGES
HepG2 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ACE2 Antibody（bs-1004R） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ACE2 Polyclonal Antibody, Unconjugated (bs-1004R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ACE2 Polyclonal Antibody, Unconjugated (bs-1004R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ACE2 Polyclonal Antibody, Unconjugated (bs-1004R) at 1:200 overnight at 4°C, DAB staining.