Host: Rabbit
Target Protein: Alpha s2 Casein
Immunogen Range: 165-203/222
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 282209
Swiss Prot: P02663
Source: KLH conjugated synthetic peptide derived from cow Alpha s2 Casein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
Predicted Molecular Weight: 24
Cross Reactive Species:
Mouse
Bovine
Predicted Cross Reactive Species:
Cow
Sheep
For research use only. Not intended for diagnostic or therapeutic use.
- Gao, H. N., et al. "Leucine and histidine independently regulate milk protein synthesis in bovine mammary epithelial cells via mTOR signaling pathway." J. Zhejiang Univ.-Sci. B (Biomed. & Biotechnol.) 16.6 (2015): 560-572.Read more>>
- Luo et al. SESN2 negatively regulates cell proliferation and casein synthesis by inhibition the amino acid-mediated mTORC1 pathway in cow mammary epithelial cells. (2018) Sci.Rep. 8:3912Read more>>
- Chiara Rossi. et al. Reaction of cysteine residues with oxidized tyrosine residues mediates cross-linking of photo-oxidized casein proteins. Food Chem. 2022 Aug;385:132667Read more>>
Paraformaldehyde-fixed, paraffin embedded bovine breast tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-alpha S2 casein Polyclonal Antibody, Unconjugated (bs-10034R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining