Host: Rabbit
Target Protein: beta casein
Immunogen Range: 16-100/224
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 281099
Swiss Prot: P02666
Source: KLH conjugated synthetic peptide derived from bovine Beta-casein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-F(1:100-500)
Predicted Molecular Weight: 24
Cross Reactive Species:
Human
Bovine
Goat
Cow
Predicted Cross Reactive Species: Sheep
For research use only. Not intended for diagnostic or therapeutic use.
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- Hou et al. Spleen tyrosine kinase regulates mammary epithelial cell proliferation in mammary glands of dairy cows. (2016) J.Dairy.Sci. 99:3858-68Read more>>
- Wang B et al. Laminin-dependent integrin β1 signaling regulates milk protein synthesis via prolactin/STAT5 pathway in bovine mammary epithelial cells. Biochem Biophys Res Commun. 2020 Jan 24. Read more>>
- Chaochao Luo. et al. Glutamine Regulates Cell Growth and Casein Synthesis through the CYTHs/ARFGAP1-Arf1-mTORC1 Pathway in Bovine Mammary Epithelial Cells. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXXRead more>>
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- Minghui Zhang. et al. Comparative Transcriptomic Analysis of Mammary Gland Tissues Reveals the Critical Role of GPR110 in Palmitic Acid-Stimulated Milk Protein and Fat Synthesis. BRIT J NUTR. 2023 Mar;:1-32Read more>>
- Xiaoyu Duan. et al. mTORC2-AKT-LAT1 Signalling Participates in Methionine-induced -CASEIN Expression in Mammary Epithelial Cells of Dairy Cows. J ANIM PHYSIOL AN N. 2023 MaRead more>>
- Xiaoming Hou. et al. Prolactin upregulates amino acids uptake in dairy cow mammary epithelial cells via LAT1. J DAIRY SCI. 2024 JuRead more>>
- Yuwen Zhou. et al. RNA-seq screening and gene function analysis uncover GPR183 as a key mediator for methionine to stimulate milk synthesis in mouse mammary epithelial cells. BRIT J NUTR. 2024 Jun;:1-29Read more>>
- Nurul Izza Nabilah Mohd Samsudin. et al. Comparative detection of goat's milk casein through various enzyme-linked immunosorbent assay (ELISA) approaches using caprine alpha-S1 casein peptides and bovine casein polyclonal antibodies. INT J FOOD SCI TECH. 2024 Jun;59(7):5181-5191Read more>>
- Jinlong Zhang. et al. Selenomethionine Promotes Milk Protein and Fat Synthesis and Proliferation of Mammary Epithelial Cells through the GPR37-mTOR-S6K1 Signaling. J AGR FOOD CHEM. 2024;XXXX(XXX):XXX-XXXRead more>>
Lane 1: Milk (Goat) Lysate at 2 ug Lane 2: Milk (Cow) Lysate at 30 ug Lane 3: Milk (Cow) Lysate at 3 ug Lane 4: Milk (Human) Lysate at 30 ug Primary: Anti-Beta-casein (bs-10032R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Cow milk probed with Rabbit Anti-Beta-casein Polyclonal Antibody, Unconjugated (bs-10032R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.