This image was generously provided by Adib Zendedel, PhD from RWTH Aachen University. Formalin-fixed and paraffin embedded rat spinal cord tissue labeled with Rabbit Anti-Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:300 followed by conjugation to a secondary antibody


Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Cryopyrin Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated (bs-10021R-A488) 1:400, 90 minutes at 37°C; DAPI (5ug/ml, blue) was used to stain the cell nuclei.


Lane 1: Raji lysates; Lane 2: MDA-MB-231 probed with Cryopyrin Polyclonal Antibody (bs-10021R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.


SiHa lysates probed with Rabbit Anti-Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.


Lane 1: Mouse Spleen lysates; Lane 2: Mouse Thymus lysates probed with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Lymph node lysates probed with Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Lane 1:Rat Thymus lysates probed with NLRP3 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Lane 1: Mouse RAW264.7 cell lysates; Lane 2: Mouse Thymus lysates probed with Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C


Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Large intestine lysates; Lane 3: Mouse Thymus cell lysates; Lane 4: Mouse Lung cell lysates; Lane 5: Rat Cerebrum lysates; Lane 6: Rat Thymus lysates; Lane 7: Rat Lung lysates; Lane 8: Human Lung cell lysates probed withCryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Jurkat cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NLRP3 Antibody(bs-10021R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


RAW264.7 (M) cells were treated with or without LPS (10 µg/ml) for 8h, 25 μg total protein per lane of cell lysates (see on figure) probed with NALP3/CIAS1 polyclonal antibody, unconjugated (bs-10021R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Purulent appendicitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Chronic inflammatory mastitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with NALP3/CIAS1 polyclonal antibody, unconjugated (bs-10021R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.