bs-10011R
[Primary Antibody]
Occludin Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Occludin
Immunogen Range: 151-250/522
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 100506658
Swiss Prot: Q16625
Source: KLH conjugated synthetic peptide derived from human Occludin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
Predicted Molecular Weight: 59
Cross Reactive Species:
Human
Mouse
Rat
Bovine
Pig
Others
Predicted Cross Reactive Species:
Dog
Cow
Sheep
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Chang L et al. Melamine causes testicular toxicity by destroying blood-testis barrier in piglets.Toxicol Lett. 2018 Oct 15;296:114-124. Read more>>
- Zhao B et al. N‐Acetyl‐d‐galactosamine prevents soya bean agglutinin‐induced intestinal barrier dysfunction in intestinal porcine epithelial cells. J Anim Physiol Anim Nutr (Berl). 2019 Apr 1.Read more>>
- Li X et al. Overexpression of lncRNA H19 changes basic characteristics and affects immune response of bovine mammary epithelial cells. PeerJ. 2019 Apr 5;7:e6715.Read more>>
- Han L et al. Beneficial Effects of Potentilla discolor Bunge Water Extract on Inflammatory Cytokines Release and Gut Microbiota in High-Fat Diet and Streptozotocin-Induced Type 2 Diabetic Mice.Nutrients. 2019 Mar 20;11(3).Read more>>
- Chen M et al. Dietary L-Tryptophan alleviated LPS-induced intestinal barrier injury by regulating tight junctions in Caco-2 cell monolayer model.Food Funct. 2019 May 22;10(5):2390-2398. Read more>>
- Qu XY et al. XingNaoJing injections protect against cerebral ischemia/reperfusion injury and alleviate blood-brain barrier disruption in rats, through an underlying mechanism of NLRP3 inflammasomes suppression. Chin J Nat Med. 2019 Jul;17(7):498-505.Read more>>
- Wang X et al. LRRC75A antisense lncRNA1 knockout attenuates inflammatory responses of bovine mammary epithelial cells. International Journal of Biological Sciences. 2020; 16(2): 251-263. Read more>>
- Xu T et al. IL-1β induces increased tight junction permeability in bovine mammary epithelial cells via the IL-1β-ERK1/2-MLCK axis upon blood-milk barrier damage.J Cell Biochem. 2018 Nov;119(11):9028-9041.Read more>>
- Chang L et al. Melamine causes testicular toxicity by destroying blood-testis barrier in piglets.Toxicol Lett. 2018 Oct 15;296:114-124. Read more>>
- Luo Q et al. Iron Overload Resulting from the Chronic Oral Administration of Ferric Citrate Impairs Intestinal Immune and Barrier in Mice. Biol Trace Elem Res. 2020 May 28.Read more>>
- Ting Zheng. et al. Combination of Enteral Nutrition and Probiotics Promote Recovery Following Ileal PouchCAnal Anastomosis in Rats. 2020 Nov 05Read more>>
- Zhang Boxun. et al. The Herbal Medicine Scutellaria-Coptis Alleviates Intestinal Mucosal Barrier Damage in Diabetic Rats by Inhibiting Inflammation and Modulating the Gut Microbiota. Evid-Based Compl Alt. 2020;2020:4568629Read more>>
- Yaqi Chang. et al. Glucagon\like peptide 2 attenuates intestinal mucosal barrier injury through the MLCK/pMLC signaling pathway in a piglet model. J Cell Physiol. 2021 Apr;236(4):3015-3032Read more>>
- Zheng Chen. et al. Glycine attenuates cerebrovascular remodeling via glycine receptor alpha 2 and vascular endothelial growth factor receptor 2 after stroke. Am J Transl Res. 2020; 12(10): 6895C6907Read more>>
- Xin-Ran Liu. et al. Radioprotective Effect of Whey Hydrolysate Peptides against -Radiation-Induced Oxidative Stress in BALB/c Mice. Nutrients. 2021 Mar;13(3):816Read more>>
- Yanan Gao. et al. Aflatoxin B1 and Aflatoxin M1 Induce Compromised Intestinal Integrity through Clathrin-Mediated Endocytosis. Toxins. 2021 Mar;13(3):184Read more>>
- Xiao Wang. et al. Oral CoreCShell Nanoparticles Embedded in Hydrogel Microspheres for the Efficient Site-Specific Delivery of Magnolol and Enhanced Antiulcerative Colitis Therapy. Acs Appl Mater Inter. 2021;XXXX(XXX):XXX-XXXRead more>>
- Caijun Zhao. et al. Aryl hydrocarbon receptor activation by Lactobacillus reuteri tryptophan metabolism alleviates Escherichia coli-induced mastitis in mice. Plos Pathog. 2021 Jul;17(7):e1009774Read more>>
- Caijun Zhao. et al. Kynurenic acid protects against mastitis in mice by ameliorating inflammatory responses and enhancing blood-milk barrier integrity. Mol Immunol. 2021 Sep;137:134Read more>>
- Juan Zhang. et al. The Regulatory Effects of Licochalcone A on the Intestinal Epithelium and Gut Microbiota in Murine Colitis. Molecules. 2021 Jan;26(14):4149Read more>>
- Xia Liu. et al. Luteolin alleviates non-alcoholic fatty liver disease in rats via restoration of intestinal mucosal barrier damage and microbiota imbalance involving in gut-liver axis. Arch Biochem Biophys. 2021 Oct;711:109019Read more>>
- Jianzhao Liao. et al. Gut microbiota disturbance exaggerates battery wastewater-induced hepatotoxicity through a gut-liver axis. Sci Total Environ. 2022 Feb;809:152188Read more>>
- Qing Li. et al. Fumonisin B1 Inhibits Cell Proliferation and Decreases Barrier Function of Swine Umbilical Vein Endothelial Cells. Toxins. 2021 Dec;13(12):863Read more>>
- Juan Zhang. et al. Licoflavone B, an isoprene flavonoid derived from licorice residue, relieves dextran sodium sulfate-induced ulcerative colitis by rebuilding the gut barrier and regulating intestinal microflora. Eur J Pharmacol. 2022 Feb;916:17473Read more>>
- Zhang Jin-Ming. et al. BRCC36 promotes intestinal mucosal barrier injury caused by BMP2 after ischemia-reperfusion via inhibiting PPAR signaling. Biosci Biotech Bioch. 2021 DecRead more>>
- Shiyun Chen. et al. Targeted delivery of Chinese herb pair-based berberine/tannin acid self-assemblies for the treatment of ulcerative colitis. J Adv Res. 2021 NovRead more>>
- Shaofeng Wu. et al. The neuroprotective effect of curcumin against ATO triggered neurotoxicity through Nrf2 and NF-B signaling pathway in the brain of ducks. Ecotox Environ Safe. 2021 Dec;228:112965Read more>>
- Jun Yan. et al. Intestinal toxicity of micro- and nano-particles of foodborne titanium dioxide in juvenile mice: Disorders of gut microbiotahost co-metabolites and intestinal barrier damage. Sci Total Environ. 2022 May;821:153279Read more>>
- D.L. Wu. et al. Dietary supplementation of free or two fat-coated sodium butyrate with varying release times on gastrointestinal development and tight junctions in preweaning Holstein calves. Anim Feed Sci Tech. 2022 Mar;285:115224Read more>>
- Fuqing Huang. et al. Nisin Z attenuates lipopolysaccharide-induced mastitis by inhibiting the ERK1/2 and p38 mitogen-activated protein kinase signaling pathways. J Dairy Sci. 2022 FebRead more>>
- Qi Wang. et al. Effect of oral administration of Limosilactobacillus reuteri on intestinal barrier function and mucosal immunity of suckling piglets. Ital J Anim Sci. 2022;21(1):612-623Read more>>
- Kexin Wang. et al. Phloretin Protects Bovine Rumen Epithelial Cells from LPS-Induced Injury. TOXINS. 2022 May;14(5):337Read more>>
- Jian Kang. et al. Ginsenoside Rg1 Mitigates Porcine Intestinal Tight Junction Disruptions Induced by LPS through the p38 MAPK/NLRP3 Inflammasome Pathway. TOXICS. 2022 Jun;10(6):285Read more>>
- Hongzeng Li. et al. Eugenol alleviated nonalcoholic fatty liver disease in rat via a gut-brain-liver axis involving glucagon-like Peptide-1. ARCH BIOCHEM BIOPHYS. 2022 May;:109269Read more>>
- Yao Li. et al. Sodium alginate and galactooligosaccharides ameliorate metabolic disorders and alter the composition of the gut microbiota in mice with high-fat diet-induced obesity. INT J BIOL MACROMOL. 2022 Aug;215:113Read more>>
- Shu-Ting Miao. et al. Oral administration of octacosanol modulates the gut bacteria and protects the intestinal barrier in ulcerative colitis mice. J FOOD BIOCHEM. 2022 Jun 23Read more>>
- Zipeng Gong. et al. Pharmacokinetic Differences of Wuji Pill Components in Normal and Chronic Visceral Hypersensitivity Irritable Bowel Syndrome Rats Attributable to Changes in Tight Junction and Transporters. FRONT PHARMACOL. 2022; 13: 948678Read more>>
- Wei Hao. et al. Ginger polysaccharides relieve ulcerative colitis via maintaining intestinal barrier integrity and gut microbiota modulation. INT J BIOL MACROMOL. 2022 Oct;219:73Read more>>
- Zhang, Yaxin. et al. Stigmasterol attenuates hepatic steatosis in rats by strengthening the intestinal barrier and improving bile acid metabolism. npj Science of Food. 2022 Aug;6(1):1-14Read more>>
- Irene Palenca. et al. N-Palmitoyl-D-Glucosamine Inhibits TLR-4/NLRP3 and Improves DNBS-Induced Colon Inflammation through a PPAR-α-Dependent Mechanism. BIOMOLECULES. 2022 Aug;12(8):1163Read more>>
- Wan Shuangxiu. et al. Deoxynivalenol damages the intestinal barrier and biota of the broiler chickens. BMC VET RES. 2022 Dec;18(1):1-1Read more>>
- Xianqiang Li. et al. Menthol nanoliposomes enhanced anti-tumor immunotherapy by increasing lymph node homing of dendritic cell vaccines. CLIN IMMUNOL. 2022 Sep;:109119Read more>>
- Wei Hao. et al. Classical prescription Huanglian Decoction relieves ulcerative colitis via maintaining intestinal barrier integrity and modulating gut microbiota. PHYTOMEDICINE. 2022 Sep;:154468Read more>>
- Ruiyue Sun. et al. Effects of Bacillus subtilis natto JLCC513 on gut microbiota and intestinal barrier function in obese rats. J APPL MICROBIOL. 2022 SepRead more>>
- Guangdong Bai. et al. Perinatal exposure to glyphosate-based herbicides impairs progeny health and placental angiogenesis by disturbing mitochondrial function. ENVIRON INT. 2022 Dec;170:107579Read more>>
- Wang Ruibiao. et al. Effects of exosomes derived from Trichinella spiralis infective larvae on intestinal epithelial barrier function. VET RES. 2022 Dec;53(1):1-11Read more>>
- Yanbo Guo. et al. Effects of Low-Ambient-Temperature Stimulation on Modifying the Intestinal Structure and Function of Different Pig Breeds. ANIMALS. 2022 Jan;12(20):274Read more>>
- Rongrong Fu. et al. Apigenin remodels the gut microbiota to ameliorate ulcerative colitis.. FRONT NUTR. 2022 Dec;9:1062961-1062961Read more>>
- Longfei Xiao. et al. Curcumin Ameliorates Age-Induced Tight Junction Impaired in Porcine Sertoli Cells by Inactivating the NLRP3 Inflammasome through the AMPK/SIRT3/SOD2/mtROS Signaling Pathway. OXID MED CELL LONGEV. 2023;2023:1708251Read more>>
- Xin Liu. et al. Alteration of gut microbiome and metabolome by Clostridium butyricum can repair the intestinal dysbiosis caused by antibiotics in mice. ISCIENCE. 2023 Feb;:10619Read more>>
VALIDATION IMAGES
Lane 1: Human A549 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human Hela cell lysates probed with Occludin Polyclonal Antibody, Unconjugated (bs-10011R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Occludin Antibody(bs-10011R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Occludin Polyclonal Antibody(bs-10011R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).