bs-10009R [Primary Antibody]
E cadherin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: E cadherin

Immunogen Range: 401-500/882


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 999

Swiss Prot: P12830

Source: KLH conjugated synthetic peptide derived from human E-cadherin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 90/97


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu H et al. Circular RNA has_circ_0003204 inhibits oxLDL-induced vascular endothelial cell proliferation and angiogenesis. Cell Signal. 2020 Jun;70:109595.Read more>>
  • Jayachandran, Muthuvel, et al. "Extracellular vesicles in urine of women with but not without kidney stones manifest patterns similar to men: a case control study." Biol Sex Differ 6.2 (2015).Read more>>
  • Neelam, Sudha, Morgan M. Brooks, and Patrick R. Cammarata. "Lenticular cytoprotection, part 2: Link between glycogen synthase kinase-3β, epithelial to mesenchymal transition, and mitochondrial depolarization." (2015) Molecular Vision.Read more>>
  • Wang, Jing, et al. "Phosphorylation-dependent regulation of ALDH1A1 by Aurora kinase A: insights on their synergistic relationship in pancreatic cancer." BMC biology 15.1 (2017): 10.Read more>>
  • Borin, Thaiz F., et al. "HET0016 decreases lung metastasis from breast cancer in immune-competent mouse model." PLoS One 12.6 (2017).Read more>>
  • Zhang, Wen-feng, et al. "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." Phytomedicine (2017).Read more>>
  • Yun, Yang, et al. "Sulfate Aerosols Promote Lung Cancer Metastasis by Epigenetically Regulating the Epithelial-to-Mesenchymal Transition (EMT)." Environmental Science & Technology (2017).Read more>>
  • Jinquan Cai Roles of transcriptional factor Snail and adhesion factor E-cadherin in clear cell renal cell carcinoma. (2013) Exp.Ther.Me. 6:1489-1493Read more>>
  • Jia et al. Steroidogenic enzyme Cyp11a1 regulates Type 2 CD8+ T cell skewing in allergic lung disease. (2013) Proc.Natl.Acad.Sci.U.S.. 110:8152-7Read more>>
  • Short et al. Influenza virus damages the alveolar barrier by disrupting epithelial cell tight junctions. (2016) Eur.Respir.. 47:954-66Read more>>
  • Liu and Xiao Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia. (2017) BMC.Ophthalmo. 17:135Read more>>
  • Hu et al. Hydroxysafflor Yellow A Ameliorates Renal Fibrosis by Suppressing TGF-β1-Induced Epithelial-to-Mesenchymal Transition. (2016) PLoS.On. 11:e0153409Read more>>
  • Wang et al. The Aurora-A-Twist1 axis promotes highly aggressive phenotypes in pancreatic carcinoma. (2017) J.Cell.Sci. 130:1078-1093Read more>>
  • Fei et al. The number of polyploid giant cancer cells and epithelial-mesenchymal transition-related proteins are associated with invasion and metastasis in human breast cancer. (2015) J.Exp.Clin.Cancer.Res. 34:158Read more>>
  • Deng et al. PEG10 plays a crucial role in human lung cancer proliferation, progression, prognosis and metastasis. (2014) Oncol.Rep. 32:2159-67Read more>>
  • Wang et al. Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. (2016) Sci.Rep. 6:36543Read more>>
  • Zhong, Y., et al. “Isolation of primitive mouse extraembryonic endoderm (pXEN) stem cell lines.” Stem Cell Research (2018); 30: 100-112.Read more>>
  • Wu, DM., et al. “Long Non-Coding RNA LINC01260 Inhibits the Proliferation, Migration and Invasion of Spinal Cord Glioma Cells by Targeting CARD11 Via the NF-κB Signaling Pathway.” Cellular Physiology and Biochemistry (2018); 48(4): 1563-1578.Read more>>
  • Kalem, K., et al. “Maternal nutrition and reproductive functions of female and male offspring.” Reproduction (2018).Read more>>
  • Lin, X., et al. “TFF3 Contributes to Epithelial-Mesenchymal Transition (EMT) in Papillary Thyroid Carcinoma Cells via the MAPK/ERK Signaling Pathway.” Journal of Cancer (2018); 9(23): 4430-4439.Read more>>
  • Guo F et al. miR-375-3p/YWHAZ/β-catenin axis regulates migration, invasion, EMT in gastric cancer cells.(2018) Clin Exp Pharmacol Physiol. Oct 24.Read more>>
  • Lin X et al. TFF3 Contributes to Epithelial-Mesenchymal Transition (EMT) in Papillary Thyroid Carcinoma Cells via the MAPK/ERK Signaling Pathway.(2018)J Cancer;Oct 31;9(23):4430-4439. Read more>>
  • Fang et al. Upregulation of long noncoding RNA CCAT1-L promotes epithelial-mesenchymal transition in gastric adenocarcinoma. (2018) Onco.Targets.Ther. 11:5647-5655Read more>>
  • Ma W et al. A vanillin derivative suppresses the growth of HT29 cells through the Wnt/β-catenin signaling pathway. Eur J Pharmacol. 2019 Apr 15;849:43-49. Read more>>
  • Zhou S et al. Helicobacter pylori infection promotes epithelial-to-mesenchymal transition of gastric cells by upregulating LAPTM4B. Biochem Biophys Res Commun. 2019 Jun 30;514(3):893-900.Read more>>
  • Li D et al. Oxygenated Polycyclic aromatic hydrocarbons (Oxy-PAHs) facilitate lung cancer metastasis by epigenetically regulating the epithelial-to-mesenchymal transition (EMT). Environ Pollut. 2019 Sep 17;255(Pt 2):113261. Read more>>
  • Xue Y et al. Chlorogenic acid attenuates cadmium-induced intestinal injury in Sprague–Dawley rats. Food Chem Toxicol. 2019 Aug 4;133:110751. Read more>>
  • Pan J et al. lncRNA JPX/miR-33a-5p/Twist1 axis regulates tumorigenesis and metastasis of lung cancer by activating Wnt/β-catenin signaling. Mol Cancer. 2020 Jan 15;19(1):9. Read more>>
  • Yan Y et al. Inhibition of TGF-β Signaling in Gliomas by the Flavonoid Diosmetin Isolated from Dracocephalum peregrinum L. Molecules. 2020 Jan 2;25(1). pii: E192. Read more>>
  • Liu H et al. Circular RNA has_circ_0003204 inhibits oxLDL-induced vascular endothelial cell proliferation and angiogenesis. Cell Signal. 2020 Jun;70:109595.Read more>>
  • Liu H et al. Anti-tubulin agent vinorelbine inhibits metastasis of cancer cells by regulating epithelial-mesenchymal transition. Eur J Med Chem. 2020 Aug 15;200:112332.Read more>>
  • Chen D et al. Sodium Propionate Attenuates the Lipopolysaccharide-Induced EpithelialCMesenchymal Transition via the PI3K/Akt/mTOR Signaling Pathway. J Agric Food Chem. 2020 Jun 17;68(24):6554-6563.Read more>>
  • Shi M et al. MicroRNA-27a targets Sfrp1 to induce renal fibrosis in diabetic nephropathy by activating Wnt/-Catenin signalling. Biosci Rep. 2020 Jun 26;40(6):BSR20192794.Read more>>
  • Francesca Salamanna. et al. Development and characterization of a novel human 3D model of bone metastasis from breast carcinoma in vitro cultured. Bone. 2021 Feb;143:115773Read more>>
  • Emelie Landh. et al. Properties of rapamycin solid lipid nanoparticles for lymphatic access through the lungs & part I: the effect of size. 2020 Aug 21Read more>>
  • Jin Dai. et al. NK6 Homeobox 2 Regulated Gastrokin-2 Suppresses Gastric Cancer Cell Proliferation and Invasion via Akt Signaling Pathway. Cell Biochem Biophys. 2021 Mar;79(1):123-131Read more>>
  • Sabine Arve-Butler. et al. Synovial fluid neutrophils in oligoarticular juvenile idiopathic arthritis have an altered phenotype and impaired effector functions. Arthritis Res Ther. 2021 Dec;23(1):1-12Read more>>
  • Qionghua Hu. et al. Long Non-Coding RNA CASC2 Overexpression Ameliorates Sepsis-Associated Acute Kidney Injury by Regulating MiR-545-3p/PPARA axis. J Surg Res. 2021 Sep;265:223Read more>>
  • Yu Guo. et al. RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF?1/Smad pathway. Int J Mol Med. 2021 Aug;48(2):1-13Read more>>
  • Shunichiro Hanai. et al. Hypoxia-induced thyroid hormone receptor expression regulates cell-cycle progression in renal tubule epithelial cells. 2021 Jun 08Read more>>
  • Xiaolan You. et al. Fibroblastic galectin-1-fostered invasion and metastasis are mediated by TGF-1-induced epithelial-mesenchymal transition in gastric cancer. Aging-Us. 2021 Jul 31; 13(14): 18464C18481Read more>>
  • Jin, Meiyuan. et al. MicroRNA-3935 promotes human trophoblast cell epithelial-mesenchymal transition through tumor necrosis factor receptor-associated factor 6/regulator of G protein signaling 2 axis. Reprod Biol Endocrin. 2021 Dec;19(1):1-15Read more>>
  • Shi, Yesi. et al. Biomimetic nanoparticles blocking autophagy for enhanced chemotherapy and metastasis inhibition via reversing focal adhesion disassembly. J Nanobiotechnol. 2021 Dec;19(1):1-17Read more>>
  • Yuhua Li. et al. Bolbostemma paniculatum (Maxim.) Franquet extract suppresses the development of colorectal cancer through downregulation of PI3K/Akt pathway. J Ethnopharmacol. 2022 Apr;287:114937Read more>>
  • Haixiang Ding. et al. Ailanthone suppresses the activity of human colorectal cancer cells through the STAT3 signaling pathway. Int J Mol Med. 2022 Feb;49(2):1-11Read more>>
  • Gurur Garip. et al. Effect of endoplasmic reticulum stress on human trophoblast cells: Survival triggering or catastrophe resulting in death. ACTA HISTOCHEM. 2022 Oct;124:151951Read more>>
  • Xiaoyan Song. et al. SRRM1 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by regulating the JAK/STAT signaling pathway. TISSUE CELL. 2022 Oct;:101954Read more>>
  • Guo, Zhi-chen. et al. Porphyromonas gingivalis promotes the progression of oral squamous cell carcinoma by activating the neutrophil chemotaxis in the tumour microenvironment. CANCER IMMUNOL IMMUN. 2022 Dec;:1-17Read more>>
  • Shugen Xu. et al. Effect of miR-21-3p on lung injury in rats with traumatic hemorrhagic shock resuscitated with sodium bicarbonate Ringers solution. ANN TRANSL MED. 2022 Dec; 10(24): 1331Read more>>
  • Xiaobei Zhang. et al. The roles and molecular mechanisms of long non-coding RNA WT1-AS in the maintenance and development of gastric cancer stem cells.. HELIYON. 2023 Mar;9(4):e14655-e14655Read more>>
VALIDATION IMAGES

Mouse splenocytes probed with Rabbit Anti-E cadherin Polyclonal Antibody, PE-Cy5.5 Conjugated (bs-10009R-PE-Cy5.5) at 1:50 for 40 minutes (green)compared to control cells (blue).


Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-E cadherin Polyclonal Antibody (bs-10009R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.


Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-E cadherin Polyclonal Antibody (bs-10009R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.


MCF-7 cells were stained with E cadherin Polyclonal Antibody, Unconjugated(bs-10009R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.


Image provided by Independent Validation badge 29768.\n\nHistogram of MCF7 cells stained with Rabbit Anti-E-Cadherin Polyclonal Antibody (orange)(bs-10009R at 1:100), isotype control antibody (green), secondary antibody only (blue) and unstained (red)


Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin/CD324 Polyclonal Antibody, Unconjugated(bs-10009R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.


Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with E cadherin Polyclonal Antibody, Unconjugated (bs-10009R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with E cadherin Polyclonal Antibody, Unconjugated (bs-10009R) at 1:400 overnight at 4°C, DAB staining.


Lane 1: Human A431 cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human HepG2 cell lysates probed with E cadherin Polyclonal Antibody, Unconjugated (bs-10009R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (E cadherin) Polyclonal Antibody, Unconjugated (bs-10009R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Incubation with (E cadherin) Polyclonal Antibody, Unconjugated (bs-10009R) at 1:200 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Incubation with (E cadherin) Polyclonal Antibody, Unconjugated (bs-10009R) at 1:200 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.