bs-10005R
[Primary Antibody]
c-Kit Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: c-Kit
Immunogen Range: 350-440/976
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3815
Swiss Prot: P10721
Source: KLH conjugated synthetic peptide derived from human c-Kit
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 105
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Cow
Sheep
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Chen, Yu-Jen, et al. "Resveratrol Inhibits Alpha-Melanocyte-Stimulating Hormone Signaling, Viability, and Invasiveness in Melanoma Cells." Evidence-Based Complementary and Alternative Medicine 2013 (2013).Read more>>
- Kennedy, Eimear, et al. "Embryonic rat vascular smooth muscle cells revisited-a model for neonatal, neointimal SMC or differentiated vascular stem cells?." Vascular Cell 6.1 (2014): 6.Read more>>
- Lin, Guohua, et al. "Effect of electroacupuncture on gastric interstitial cells of Cajal in a rat model of diabetic gastroparesis." Experimental and Therapeutic Medicine.Read more>>
- Tian Li. et al. Laxative effect and mechanism of Tiantian Capsule on loperamide-induced constipation in rats. J Ethnopharmacol. 2021 Feb;266:113411Read more>>
- Yifan Yuan. et al. A Pulmonary Vascular Model From Endothelialized Whole Organ Scaffolds. Front Bioeng Biotech. 2021; 9: 760309Read more>>
- Tetsuo Miyake. et al. Genetic deletion of osteoprotegerin attenuates asthma development through suppression of inflammatory response in mice. CELL IMMUNOL. 2022 Aug;378:104559Read more>>
- Xu-Ping JIANG. et al. Youjing granules ameliorate spermatogenesis in rats through regulating the prolifereation of spermatogonial stem cells. CHIN J NAT MEDICINES. 2022 Aug;20:58Read more>>
- Yang Dapeng. et al. Morphological and histochemical identification of telocytes in adult yak epididymis. SCI REP-UK. 2023 Mar;13(1):1-14Read more>>
- Qianyu Cheng. et al. Establishing and characterizing human stem cells from the apical papilla immortalized by hTERT gene transfer. FRONT CELL DEV BIOL. 2023; 11: 1158936Read more>>
- Jie Su. et al. Study of spermatogenic and Sertoli cells in the Hu sheep testes at different developmental stages. FASEB J. 2023 Jul;37(8):e23084Read more>>
- Xiaofan Xiong. et al. Cell apoptosis in the testis of male rats is elevated by intervention with -endorphin and the mu opioid receptor. REPROD BIOL. 2023 Sep;23:100789Read more>>
- Liu Xinfeng. et al. Evolutionary origin of genomic?structural variations in domestic yaks. NAT COMMUN. 2023 Sep;14(1):1-14Read more>>
- Kuang-Shun Chueh. et al. Low-Intensity Extracorporeal Shock Wave Therapy Ameliorates Detrusor Hyperactivity with Impaired Contractility via Transient Potential Vanilloid Channels: A Rat Model for Ovarian Hormone Deficiency. INT J MOL SCI. 2024 Jan;25(9):4927Read more>>
- Rui Yang. et al. H3K9me3 Levels Affect the Proliferation of Bovine Spermatogonial Stem Cells. INT J MOL SCI. 2024 Jan;25(17):9215Read more>>
- Hongjuan Wang. et al. Differentiation of cultured hair follicle neural crest stem cells into functional melanocytes. HELIYON. 2024 Aug;1Read more>>
VALIDATION IMAGES
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-c-Kit Polyclonal Antibody (bs-10005R) at 1:200 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Formalin-fixed and paraffin embedded rat intestine labeled with Rabbit Anti-c-Kit Polyclonal Antibody, Unconjugated (bs-10005R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-c-Kit Polyclonal Antibody (bs-10005R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded Mouse ovarian; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with c-Kit Polyclonal Antibody, Unconjugated (bs-10005R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat ovarian; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with c-Kit Polyclonal Antibody, Unconjugated (bs-10005R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with c-Kit Polyclonal Antibody, Unconjugated (bs-10005R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with c-Kit Polyclonal Antibody, Unconjugated (bs-10005R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with c-Kit Polyclonal Antibody, Unconjugated (bs-10005R) at 1:200 overnight at 4°C, DAB staining.