bs-0988R-Cy5.5 [Conjugated Primary Antibody]
Transferrin receptor Polyclonal Antibody, Cy5.5 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Transferrin receptor

Immunogen Range: 621-720/760


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7037

Swiss Prot: P02786

Source: KLH conjugated synthetic peptide derived from human Transferrin receptor

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.

Conjugation: Cy5.5

Excitation/ Emission: 675nm/694nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)

Predicted Molecular Weight: 74/85


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Horse
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Song Q et al. Astragaloside IV protects against retinal iron overload toxicity through iron regulation and the inhibition of MAPKs and NF-B activationToxicol Appl Pharmacol.2021 Jan 1;410:115361.Read more>>
  • Mingang Hao. et al. Autophagy Blockade Limits HER2+ Breast Cancer Tumorigenesis by Perturbing HER2 Trafficking and Promoting Release Via Small Extracellular Vesicles. Dev Cell. 2021 Feb;56:341Read more>>
  • Sarah Almas. et al. Cytokine trafficking of IL\9 and IL\13 through TfnRc+ vesicles in activated human eosinophils. 2020 Sep 1Read more>>
  • Das Oishika. et al. Butyrate driven raft disruption trots off enteric pathogen invasion: possible mechanism of colonization resistance. GUT PATHOG. 2023 Dec;15(1):1-14Read more>>
  • Limin Shi. et al. FoxO1 regulates adipose transdifferentiation and iron influx by mediating Tgf1 signaling pathway. REDOX BIOL. 2023 Jul;63:102727Read more>>
  • Yu-Sheng Shi. et al. Dendrobine rescues cognitive dysfunction in diabetic encephalopathy by inhibiting ferroptosis via activating Nrf2/GPX4 axis. PHYTOMEDICINE. 2023 Jul;:154993Read more>>
  • Huan Yang. et al. Heat stress induces ferroptosis of porcine Sertoli cells by enhancing CYP2C9-Ras- JNK axis. THERIOGENOLOGY. 2024 Feb;215:281Read more>>
  • Jing Zhao. et al. Differentiation of intestinal stem cells toward goblet cells under systemic iron overload stress are associated with inhibition of Notch signaling pathway and ferroptosis. REDOX BIOL. 2024 Jun;72:10316Read more>>
  • Cuiping Xu. et al. Comparison of the components of fresh Panax notoginseng processed by different methods and their anti-anemia effects on cyclophosphamide-treated mice. J ETHNOPHARMACOL. 2024 Apr;:118148Read more>>
VALIDATION IMAGES