bs-0982R [Primary Antibody]
NFKB p65(Ser536) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NFKB p65 Ser536

Modification Site: Ser536

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5970

Swiss Prot: Q04206

Source: KLH conjugated synthetic phosphopeptide derived from human NFKBp65 around the phosphorylation site of(Ser536)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)

Predicted Molecular Weight: 61


Cross Reactive Species: Human
Mouse
Rat
Bovine
Chicken

Predicted Cross Reactive Species: Dog
Cow
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang X et al. trans-Cinnamaldehyde Reverses Depressive-Like Behaviors in Chronic Unpredictable Mild Stress Rats by Inhibiting NF-κB/NLRP3 Inflammasome Pathway. Evid Based Complement Alternat Med. 2020 Feb 28;2020:4572185.Read more>>
  • Zhao, Hongyu, et al. "Betulin attenuates lung and liver injuries in sepsis."International Immunopharmacology 30 (2016): 50-56.Read more>>
  • Fu, Juanli, et al. "Tetrachlorobenzoquinone exhibits neurotoxicity by inducing inflammatory responses through ROS-mediated IKK/IκB/NF-κB signaling."Environmental Toxicology and Pharmacology (2016).Read more>>
  • Zhao, Hongyu, et al. "Betulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-κB signaling pathway."Life Sciences (2016).Read more>>
  • Zhang, Liang, et al. "Knockdown of placental growth factor (PLGF) mitigates hyperoxia-induced acute lung injury in neonatal rats: Suppressive effects on NFκB signaling pathway." International Immunopharmacology 38 (2016): 167-174.Read more>>
  • Gao, Wenyan, et al. "Indirubin ameliorates dextran sulfate sodium-induced ulcerative colitis in mice through the inhibition of inflammation and the induction of Foxp3-expressing regulatory T cells." Acta Histochemica (2016).Read more>>
  • Sun, Guodong, et al. "Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats." Experimental Animals: 16-0102.Read more>>
  • Ge, Lingqing, et al. "Design and discovery of novel thiazole derivatives as potential MMP inhibitors to protect against acute lung injury in sepsis rats via attenuation of inflammation and apoptotic oxidative stress." RSC Advances7.52 (2017): 32909-32922.Read more>>
  • Singh, Anand, et al. "NADPH oxidase 4 modulates hepatic responses to lipopolysaccharide mediated by Toll-like receptor-4." Scientific Reports 7.1 (2017): 14346.Read more>>
  • Liu, Qiang, et al. "Valproic acid attenuates inflammation of optic nerve and apoptosis of retinal ganglion cells in a rat model of optic neuritis." Biomedicine & Pharmacotherapy(2017).Read more>>
  • Yang et al. Celastrol Attenuates Multiple Sclerosis and Optic Neuritis in an Experimental Autoimmune Encephalomyelitis Model. (2017) Front.Pharmacol. 8:44Read more>>
  • Chen D et al. Increasing hypothalamic nucleobindin 2 levels and decreasing hypothalamic inflammation in obese male mice via diet and exercise alleviate obesity-associated hypogonadism. (2018) Neuropeptides.Nov 1. pii: S0143-4179(18)30147-1. Read more>>
  • Li X et al. Overexpression of lncRNA H19 changes basic characteristics and affects immune response of bovine mammary epithelial cells. PeerJ. 2019 Apr 5;7:e6715.Read more>>
  • Liu F et al. GABAB receptor activation attenuates inflammatory orofacial pain by modulating interleukin-1β in satellite glial cells: role of NF-κB and MAPK signaling pathways. Brain Res Bull. 2019 Apr 26;149:240-250.Read more>>
  • Wang G et al. Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blockingthe TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response. Oxid Med Cell Longev. 2019 Apr 28;2019:7850324.Read more>>
  • Peng LY et al. Baicalin alleviated APEC-induced acute lung injury in chicken by inhibiting NF-κB pathwayactivation. Int Immunopharmacol. 2019 Jul;72:467-472.Read more>>
  • Song X et al. Anti-aging effects exerted by Tetramethylpyrazine enhances self-renewal and neuronal differentiation of rat bMSCs by suppressing NF-kB signaling. Biosci Rep. 2019 Jun 25;39(6). pii: BSR20190761. Read more>>
  • Zhang H et al. Dexmedetomidine ameliorates LPS induced acute lung injury via GSK-3β/STAT3-NF-κB signaling pathway in rats. Int Immunopharmacol. 2019 Jun 26;74:105717. Read more>>
  • Chen Y et al. Dexmedetomidine protects against lipopolysaccharide-induced early acute kidney injury by inhibiting the iNOS/NO signaling pathway in rats. Nitric Oxide. 2019 Apr 1;85:1-9. Read more>>
  • Lei J et al. LncRNA SNHG1 Alleviates IL-1β-induced Osteoarthritis by Inhibiting miR-16-5p-mediated p38 MAPK and NF-κB Signaling Pathways. Biosci Rep. 2019 Aug 5. pii: BSR20191523.Read more>>
  • Wu ZY et al. Baicalin Attenuates Mycoplasma gallisepticum-Induced Inflammation via Inhibition of the TLR2-NF-κB Pathway in Chicken and DF-1 Cells. Infection and Drug Resistance 2019:12 3911–3923.Read more>>
  • Wang X et al. LRRC75A antisense lncRNA1 knockout attenuates inflammatory responses of bovine mammary epithelial cells. International Journal of Biological Sciences. 2020; 16(2): 251-263. Read more>>
  • Peng LY et al. Protective Effect of Piceatannol Against Acute Lung Injury Through Protecting the Integrity of Air-Blood Barrier and Modulating the TLR4/NF-κB Signaling Pathway Activation. Front Pharmacol. 2020 Jan 22;10:1613.Read more>>
  • Gao X et al. Inhibition of LOX-1 prevents inflammation and photoreceptor cell death in retinal degeneration. Int Immunopharmacol. 2020 Mar;80:106190. Read more>>
  • Zou P et al. Protective effect of isoliquiritigenin against cerebral injury in septic mice via attenuation of NF-κB.Inflammopharmacology. 2018 Jun 26.Read more>>
  • Zhai W et al. Paeoniflorin inhibits Rho kinase activation in joint synovial tissues of rats with collagen-induced rheumatoid arthritis.Biomed Pharmacother. 2018 Oct;106:255-259.Read more>>
  • Liu W et al. Glycyrrhizic acid from licorice down-regulates inflammatory responses via blocking MAPK and PI3K/Akt-dependent NF-κB signalling pathways in TPA-induced skin inflammation. MedChemComm. 2018.Read more>>
  • Wei X et al. Nerve growth factor promotes ASIC1a expression via the NF-κB pathway and enhances acid-induced chondrocyte apoptosis. Int Immunopharmacol. 2020 Mar 5;82:106340. Read more>>
  • Wang X et al. trans-Cinnamaldehyde Reverses Depressive-Like Behaviors in Chronic Unpredictable Mild Stress Rats by Inhibiting NF-κB/NLRP3 Inflammasome Pathway. Evid Based Complement Alternat Med. 2020 Feb 28;2020:4572185.Read more>>
  • Peng L et al. Madecassoside Protects Against LPS-Induced Acute Lung Injury via Inhibiting TLR4/NF-B Activation and Blood-Air Barrier Permeability. Front Pharmacol. 2020 Jun 5;11:807.Read more>>
  • Xu D et al. Saturated fatty acids activate the inflammatory signalling pathway in Schwann cells: Implication in sciatic nerve injury. Scand J Immunol. 2020 Aug;92(2):e12896.Read more>>
  • Cuicui Zhuanget al. Selenomethionine Suppressed TLR4/NF-B Pathway by Activating Selenoprotein S to Alleviate ESBL Escherichia coli-Induced Inflammation in Bovine Mammary Epithelial Cells and Macrophages. Front Microbiol. 2020 Jul 8;11:1461.Read more>>
  • Shunichi Oka. et al. A deficiency of Dec2 triggers periodontal inflammation and pyroptosis. 2021 Feb 28Read more>>
  • Jian Wang. et al. Lactobacillus salivarius ameliorated Mycoplasma gallisepticum-induced inflammatory injury and secondary Escherichia coli infection in chickens: Involvement of intestinal microbiota. Vet Immunol Immunop. 2021 Mar;233:110192Read more>>
  • Dong Zhixia. et al. Adiponectin Inhibits NLRP3 Inflammasome Activation in Nonalcoholic Steatohepatitis via AMPK-JNK/ErK1/2-NFB/ROS Signaling Pathways. Front Med-Lausanne. 2020 Nov;7:705Read more>>
  • Lumeng Niu. et al. Puerarin inhibits Mycoplasma gallisepticum (MG-HS)-induced inflammation and apoptosis via suppressing the TLR6/MyD88/NF-B signal pathway in chicken. Int Immunopharmacol. 2020 Nov;88:106993Read more>>
  • Wenbo Ge. et al. 17-estradiol protects sheep oviduct epithelial cells against lipopolysaccharide-induced inflammation in vitro. Mol Immunol. 2020 Nov;127:21Read more>>
  • Zheng XP. et al. Kidney-targeted baicalin-lysozyme conjugate ameliorates renal fibrosis in rats with diabetic nephropathy induced by streptozotocin.. Bmc Nephrol. 2020 May;21(1):174-174Read more>>
  • Chen Siyuan. et al. Serine Supports IL-1 Production in Macrophages Through mTOR Signaling. Front Immunol. 2020 Aug;11:1866Read more>>
  • Cntia Yuki Fukuoka. et al. Early effect of laser irradiation in signaling pathways of diabetic rat submandibular salivary glands. Plos One. 2020 Aug;15(8):e0236727Read more>>
  • Xuting Liu. et al. Amorphous silica nanoparticles induce inflammation via activation of NLRP3 inflammasome and HMGB1/TLR4/MYD88/NF-kb signaling pathway in HUVEC cells. J Hazard Mater. 2021 Feb;404:12405Read more>>
  • Amitha Muraleedharan. et al. Protein kinase C eta is activated in reactive astrocytes of an Alzheimer's disease mouse model: Evidence for its immunoregulatory function in primary astrocytes. Glia. 2021 Mar;69(3):697-714Read more>>
  • Sijie Yuan. et al. Bacteroides vulgatus diminishes colonic microbiota dysbiosis ameliorating lumbar bone loss in ovariectomized mice. Bone. 2021 Jan;142:11571Read more>>
  • Changwei Zou. et al. Potential hazardous effects of printing room PM2.5 exposure include promotion of lung inflammation and subsequent injury. Mol Med Rep. 2020 Oct;22(4):3213-3224Read more>>
  • Jian Wang. et al. A respiratory commensal bacterium acts as a risk factor for Mycoplasma gallisepticum infection in chickens. Vet Immunol Immunop. 2020 Dec;230:110127Read more>>
  • Feng Gao. et al. Aucubin ameliorates the LPS\induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF\B and activating the Keap1/Nrf2 signalling pathway. 2021 Apr 27Read more>>
  • Qihe Tang. et al. Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-B Pathways Against Klebsiella pneumonia Infection. Front Pharmacol. 2021; 12: 651664Read more>>
  • Xiuhong Wang. et al. Protective effect of combination of anakinra and MCC950 against acute lung injury is achieved through suppression of the NF-B-mediated-MAPK and NLRP3-caspase pathways. Int Immunopharmacol. 2021 Aug;97:107506Read more>>
  • Xiaoyue Guan. et al. Crosstalk between Wnt/-catenin signaling and NF-B signaling contributes to apical periodontitis. Int Immunopharmacol. 2021 Sep;98:107843Read more>>
  • Hai-Ming Guo. et al. Spinal Ninjurin2 contributes to the neuropathic pain via NF-B-mediated neuroinflammation in the spared sciatic nerve injury rats. Int Immunopharmacol. 2021 Oct;99:107918Read more>>
VALIDATION IMAGES

Mouse spleen lysates probed with Rabbit Anti-NFKB p65(Ser536) Polyclonal Antibody, Unconjugated (bs-0982R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.


Mouse spleen lysates probed with Rabbit Anti-NFKB p65(Ser536) Polyclonal Antibody, Unconjugated (bs-0982R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT


Mouse lung lysates probed with Rabbit Anti-NFkB p65 (Ser536) Polyclonal Antibody, Unconjugated (bs-0982R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.


HL-60 cells probed with NFKB p65(Ser536) Polyclonal Antibody, unconjugated (bs-0982R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)


Lane 1: Human MOLT-4 cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human Jurkat cell lysates probed with NFKB p65 Polyclonal Antibody, Unconjugated (bs-0982R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human Siha cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human A431 cell lysates probed with NFKB p65 Polyclonal Antibody, Unconjugated (bs-0982R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human MCF-7 cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human HL-60 cell lysates; Lane 4: Mouse RAW264.7 cell lysates; Lane 5: Mouse NIH/3T3 cell lysates; Lane 6:Human Jurkat cell lysates; Lane 7: Human A549 cell lysates probed with NFKB p65 Polyclonal Antibody, Unconjugated (bs-0982R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Large intestine lysates; Lane 2: Rat Large intestine lysates; Lane 3: Mouse Lung lysates; Lane 4: Rat Lung lysates; Lane 5: Mouse Urinary bladder lysates; Lane 6: Mouse RAW264.7 cell lysates probed with NFKB p65 Polyclonal Antibody, Unconjugated (bs-0982R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


NIH/3T3 (M) cells were treated with or without Calyculin A (100nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-NFKB p65 polyclonal antibody, unconjugated (bs-0982R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.