bs-0966R
[Primary Antibody]
HA tag Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: HA tag
Clonality: Polyclonal
Isotype: IgG
Source: KLH conjugated HA Tag peptide
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
Cross Reactive Species:
Tags
Others
(HA tag Fusion Protein)
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Pop, Marius S., Dina Wassaf, and Angela N. Koehler. "Probing Small‐Molecule Microarrays with Tagged Proteins in Cell Lysates." Current protocols in chemical biology (2013): 209-220.Read more>>
- Xusheng Du. et al. RIOK3-mediated Akt phosphorylation facilitates synergistic replication of Mareks disease and reticuloendotheliosis viruses. VIRULENCE. 2022 Jul 06Read more>>
- Guo Jing. et al. Engineered minimal type I CRISPR-Cas system for transcriptional activation and base editing in human cells. NAT COMMUN. 2024 Aug;15(1):1-16Read more>>
VALIDATION IMAGES
Lane 1: E.coli Lysate lysates probed with HA tag Polyclonal Antibody, Unconjugated (bs-0966R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HA-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 4 ug Primary: Anti-HA tag (bs-0966R) at 1/1000 ~ 1/10000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
8ug tags Positive Control Whole Cell Lysate (bs-41230L) per lane probed with HA Tag polyclonal antibody respectively, HRP conjugated (bs-0966R-HRP) at 1:50000 dilution and 4°C overnight incubation.