bs-0963R
[Primary Antibody]
MTNR1B Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: MTNR1B
Immunogen Range: 201-300/362
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4544
Swiss Prot: P49286
Source: KLH conjugated synthetic peptide derived from human MTNR1B
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background:
High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 40
Cross Reactive Species:
Human
Mouse
Rat
Goat
Predicted Cross Reactive Species: Dog
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wang, S-J., et al. "Melatonin suppresses apoptosis and stimulates progesterone production by bovine granulosa cells via its receptors (MT1 and MT2)." Theriogenology 78.7 (2012): 1517-1526.Read more>>
- Brockus, K. E., et al. "Dietary melatonin alters uterine artery hemodynamics in pregnant Holstein heifers." Domestic Animal Endocrinology (2015).Read more>>
- Jang, Hoon, et al. "Synergistic effect of melatonin and ghrelin in preventing cisplatin‐induced ovarian damage via regulation of FOXO3a phosphorylation and binding to the p27Kip1 promoter in primordial follicles." Journal of Pineal Research (2017).Read more>>
- Zhang W et al. The inhibitory effect of melatonin on mammary function of lactating dairy goats.(2018) Biol Reprod. Oct 22.Read more>>
- Gao Y et al. Melatonin receptor depletion suppressed hCG-induced testosterone expression in mouse Leydig cells.Cell Mol Biol Lett. 2019 Mar 12;24:21.Read more>>
- Zhao Shu-Qin. et al. The in vitro effects of melatonin and Cry gene on the secretion of estradiol from camel ovarian granulosa cells. Domest Anim Endocrin. 2021 Jan;74:106497Read more>>
- S-Q. Zhao. et al. cAMP/PKA/CREB signaling pathway-mediated effects of melatonin receptor genes on clock gene expression in Bactrian camel ovarian granulosa cells. Domest Anim Endocrin. 2021 Jul;76:106609Read more>>
- Donghui Yang. et al. Melatonin alleviates LPS\induced endoplasmic reticulum stress and inflammation in spermatogonial stem cells. J Cell Physiol. 2021 May;236(5):3536-3551Read more>>
- Shanshan Yu. et al. Melatonin prevents experimental central serous chorioretinopathy in rats. J PINEAL RES. 2022 Apr 29Read more>>
- Jie Yang. et al. Exposure to Benzo(a)pyrene damages mitochondrial function via suppressing mitochondrial melatonin receptors in ovarian corpus luteum during early pregnancy. CHEM-BIOL INTERACT. 2022 Aug;:110085Read more>>
- Wenbo Ge. et al. Melatonin protects sheep endometrial epithelial cells against lipopolysaccharide-induced inflammation in vitro. REPROD DOMEST ANIM. 2022 AugRead more>>
- Hongwei Duan. et al. Melatonin regulates dihydrotestosterone formation via its membrane receptor in the epididymal epithelial cells of sheep. THERIOGENOLOGY. 2022 DecRead more>>
- Junyuan Ma. et al. Effects of melatonin on development and hormone secretion of sheep theca cells in?vitro. THERIOGENOLOGY. 2023 Mar;198:172Read more>>
- Gao Xinran. et al. Role of Bmal1 in Type 2 Diabetes Mellitus-Related Glycolipid Metabolic Disorder and Neuropsychiatric Injury: Involved in the Regulation of Synaptic Plasticity and Circadian Rhythms. MOL NEUROBIOL. 2023 May;:1-23Read more>>
- Yunxian Dong. et al. Melatonin inhibits fibroblast cell functions and hypertrophic scar formation by enhancing autophagy through the MT2 receptor-inhibited PI3K/Akt /mTOR signaling. BBA-MOL BASIS DIS. 2024 Jan;1870:166887Read more>>
- Hongwei Duan. et al. Melatonin promotes progesterone secretion in sheep luteal cells by regulating autophagy via the AMPK/mTOR pathway. THERIOGENOLOGY. 2023 NovRead more>>
- Naisheng Lu. et al. Effect of Day Length on Growth and Gonadal Development in Meishan Male Pigs. ANIMALS. 2024 Jan;14(6):876Read more>>
- Xinran Gao. et al. Protective effect of melatonin against metabolic disorders and neuropsychiatric injuries in type 2 diabetes mellitus mice. PHYTOMEDICINE. 2024 Aug;131:155805Read more>>
- Shiyin Li. et al. Homeostatic Shrinkage of Dendritic Spines Requires Melatonin Type 3 Receptor Activation During Sleep. ADV SCI. 2024 Aug;:2400253Read more>>
- Yaling Wang. et al. Melatonin targets the paraventricular thalamus to promote non-rapid eye movement sleep in C3H/HeJ mice. CURR BIOL. 2024 02Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Anti-MTNR1B Polyclonal Antibody, Unconjugated (bs-0963R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat retina labeled with Anti-MTNR1B Polyclonal Antibody, Unconjugated (bs-0963R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
HepG2 cell lysates probed with MTNR1B Polyclonal Antibody, Unconjugated (bs-0963R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (MTNR1B) polyclonal Antibody, Unconjugated (bs-0963R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (Mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTNR1B) Polyclonal Antibody, Unconjugated (bs-0963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MTNR1B Polyclonal Antibody, Unconjugated (bs-0963R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MTNR1B Polyclonal Antibody, Unconjugated (bs-0963R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with MTNR1B polyclonal antibody, unconjugated (bs-0963R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.