bs-0908R
[Primary Antibody]
JAK2 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: JAK2
Immunogen Range: 601-700/1132
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3717
Swiss Prot: O60674
Source: KLH conjugated synthetic peptide derived from human JAK2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 131
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wei, Yanfei, et al. "Plumbagin Inhibits Leptin-Induced Proliferation of Hepatic Stellate Cells via JAK2-STAT3 Pathway to Protect against Hepatic Fibrosis."Tropical Journal of Pharmaceutical Research?12.5 (2013): 691-698.Read more>>
- Park GB et al. MicroRNA-503-5p Inhibits the CD97-Mediated JAK2/STAT3 Pathway in Metastatic or Paclitaxel-Resistant Ovarian Cancer Cells. Neoplasia. 2019 Feb;21(2):206-215.Read more>>
- Li ZH et al. You-Gui-Yin improved the reproductive dysfunction of male rats with chronic kidney disease via regulating the HIF1α-STAT5 pathway. J Ethnopharmacol. 2020 Jan 10;246:112240.Read more>>
- Xu J et al. Regulatory effect of two Trichinella spiralis serine protease inhibitors on the host's immune system. Sci Rep. 2019 Nov 19;9(1):17045.Read more>>
- Lei Yuet al. TMF, a natural dihydroflavonoid isolated from Scutellaria javanica Jungh, stimulates anticancer activity of s180 cancer-bearing mice, induces apoptosis, inhibits invasion and migration on HepG-2?cells. J Ethnopharmacol. 2020 Dec 5;263:113072.Read more>>
- Zhuo Han. et al. New insights into Vitamin C function: Vitamin C induces JAK2 activation through its receptor-like transporter SVCT2. Int J Biol Macromol. 2021 Mar;173:379Read more>>
- Ashok Kumar Jayavelu. et al. Splicing factor YBX1 mediates persistence of JAK2 -mutated neoplasms. Nature. 2020 Nov;588(7836):157-163Read more>>
- Jisheng Wang. et al. Biological Network Model of Effect of Chronic Intermittent Hypoxia on Spermatogenesis in Rats. Med Sci Monitor. 2020; 26: e925579-1Ce925579-11Read more>>
- Zhou Wan. et al. The Effects of RBP4 and Vitamin D on the Proliferation and Migration of Vascular Smooth Muscle Cells via the JAK2/STAT3 Signaling Pathway. Oxid Med Cell Longev. 2022;2022:3046777Read more>>
- Wang Yaxin. et al. Overexpression of estrogen receptor inhibits cellular functions of human hepatic stellate cells and promotes the anti-fibrosis effect of calycosin via inhibiting STAT3 phosphorylation. BMC PHARMACOL TOXICO. 2022 Dec;23(1):1-13Read more>>
- Yong Wang. et al. An integrated network pharmacology approach reveals that Darutigenol reduces inflammation and cartilage degradation in a mouse collagen-induced arthritis model by inhibiting the JAK-STAT3 pathway. J ETHNOPHARMACOL. 2023 May;:116574Read more>>
- Hu Bowen. et al. Local GHR roles in regulation of mitochondrial function through mitochondrial biogenesis during myoblast differentiation. CELL COMMUN SIGNAL. 2023 Dec;21(1):1-18Read more>>
- Gao Xiaoli. et al. Molecular characterization and function of JAK/STAT pathway in IPEC-J2 cells during Clostridium perfringens beta2 toxin stimulation. VET RES COMMUN. 2023 Jul;:1-8Read more>>
- Ting Xiao. et al. Ameliorative effect of Alangium chinense (Lour.) Harms on rheumatoid arthritis by reducing autophagy with targeting regulate JAK3-STAT3 and COX-2 pathways. J ETHNOPHARMACOL. 2023 Sep;:117133Read more>>
- Fuyi Luo. et al. Simultaneous blastic plasmacytoid dendritic cell neoplasm and myelofibrosis: A case report. ONCOL LETT. 2024 May;27(5):1-14Read more>>
VALIDATION IMAGES
Mouse splenocytes probed with Rabbit Anti-JAK2 Polyclonal Antibody, Unconjugated (bs-0908R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with JAK2 Polyclonal Antibody(bs-0908R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with JAK2 Polyclonal Antibody, Unconjugated (bs-0908R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Human K562 cell lysates; Lane 2: Mouse BV-2 cell lysates probed with JAK2 Polyclonal Antibody, Unconjugated (bs-0908R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with JAK2 Polyclonal Antibody, Unconjugated (bs-0908R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with JAK2 Polyclonal Antibody, Unconjugated (bs-0908R) at 1:200 overnight at 4°C, DAB staining.