bs-0890R-BF594 [Conjugated Primary Antibody]
GFP Polyclonal Antibody, AbBy Fluor® 594 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GFP

Immunogen Range: 25-120/238


Clonality: Polyclonal

Isotype: IgG

Swiss Prot: P42212

Source: KLH conjugated synthetic peptide derived from jellyfish Green Fluorecent protein

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

Conjugation: AbBy Fluor® 594

Excitation/ Emission: 590nm/617nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 27


Cross Reactive Species: Pig
Tags
(Aequorea victoria, Aequorea coerulescens (belt jellyfish))

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Galeano Niño JL et al. The Lifeact-EGFP mouse is a translationally controlled fluorescent reporter of T cell activation. J Cell Sci. 2020 Mar 5;133(5). Read more>>
  • Liu Y et al. The synergistic effect of NELL1 and adipose-derived stem cells on promoting bone formation in osteogenesis imperfecta treatment. Biomed Pharmacother. 2020 Aug;128:110235.Read more>>
  • Karim Rahimi. et al. Evaluation of miR-302 promoter activity in transgenic mice and pluripotent stem cell lines. In Vitro Cell Dev-An. 2020 Dec;56(10):896-905Read more>>
  • Ridone Pietro. et al. Disruption of membrane cholesterol organization impairs the activity of PIEZO1 channel clusters. J Gen Physiol. 2020 Aug;152(8):e201912515.Read more>>
  • Jin Chaofan. et al. Dissecting the dynamic cellular transcriptional atlas of adult teleost testis development throughout the annual reproductive cycle. DEVELOPMENT. 2024 Mar;151(20)Read more>>
VALIDATION IMAGES