bs-0878R
[Primary Antibody]
VE Cadherin Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: VE Cadherin
Immunogen Range: 601-700/784
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 12562
Swiss Prot: P55284
Source: KLH conjugated synthetic peptide derived from mouse vascular endothelial cadherin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 86
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Xia W et al. Inhibition of MRP4 alleviates sepsis-induced acute lung injury in rats. Int Immunopharmacol. 2019 Apr 14;72:211-217.Read more>>
- Bhagwani AR et al. Endothelial cells are a source of Nestin expression in Pulmonary Arterial Hypertension.PLoS One. 2019 Mar 18;14(3):e0213890.Read more>>
- Zhao F et al. Hypoxia Preconditioned Adipose Derived Endothelial Progenitor Cells Promote Bladder Augmentation. Tissue Eng Part A. 2019 Jun 26.Read more>>
- Zhang C et al. Superparamagnetic iron oxide (SPIO) nanoparticles labeled endothelial progenitor cells (EPCs) administration inhibited heterotopic ossification in rats. Nanomedicine. 2019 Aug 7;21:102078.Read more>>
- Ling-Kun Zhang. et al. Growth factors regional patterned and photoimmobilized scaffold applied to bone tissue regeneration. J Mater Chem B. 2020 Dec;8(48):10990-11Read more>>
- Jie Mi. et al. Calcitonin Gene-Related Peptide Enhances Distraction Osteogenesis by Increasing Angiogenesis. 2020 Nov 02Read more>>
- Wan\Ting Hung. et al. Leptin protects brain from ischemia/reperfusion\induced infarction by stabilizing the bloodCbrain barrier to block brain infiltration by the blood\borne neutrophils. Eur J Neurosci. 2020 Dec;52(12):4890-4907Read more>>
- Yi Ren. et al. Pravastatin attenuates sepsis-induced acute lung injury through decreasing pulmonary microvascular permeability via inhibition of Cav-1/eNOS pathway. Int Immunopharmacol. 2021 Nov;100:108077Read more>>
- Hong Yu. et al. Sustained Release of MiR-217 Inhibitor by Nanoparticles Facilitates MSC-Mediated Attenuation of Neointimal Hyperplasia After Vascular Injury. Front Cardiovasc Med. 2021; 8: 739107Read more>>
- ingcheng Yi. et al. Step-wise CAG@PLys@PDA-Cu2+ modification on micropatterned nanofibers for programmed endothelial healing. BIOACT MATER. 2022 JulRead more>>
- Xiong, Jingjie. et al. CircRNA mmu_circ_0000021 regulates microvascular function via the miR-143-3p/NPY axis and intracellular calcium following ischemia/reperfusion injury. CELL DEATH DISCOV. 2022 Jul;8(1):1-13Read more>>
- Yi, Bingcheng. et al. Soft nanofiber modified micropatterned substrates enhance native-like endothelium maturation via CXCR4/calcium-mediated actin cytoskeleton assembly. NANO RES. 2022 Aug;:1-18Read more>>
- Xin Liu. et al. Construction of functional magnetic scaffold with temperature control switch for long-distance vascular injury. BIOMATERIALS. 2022 Oct;:121862Read more>>
- Shunnosuke Kunoki. et al. Inhibition of transglutaminase 2 reduces peritoneal injury in a chlorhexidine-induced peritoneal fibrosis model. LAB INVEST. 2023 Jan;:10005Read more>>
- Li-Juan You. et al. Schisandrin A ameliorates increased pulmonary capillary endothelial permeability accompanied with sepsis through inhibition of RhoA/ROCK1/MLC pathways. INT IMMUNOPHARMACOL. 2023 May;118:110124Read more>>
- Paola Franco. et al. V-Integrin-Dependent Inhibition of Glioblastoma Cell Migration, Invasion and Vasculogenic Mimicry by the uPAcyclin Decapeptide. CANCERS. 2023 Jan;15(19):4775Read more>>
- Yuemei Yan. et al. Dangui Huoxue Preparation (DHP) Ameliorates Skin Fibrosis, Inflammation, and Vasculopathy in the Bleomycin-Induced Murine Model of Systemic Sclerosis. Advanced Biology. 2023 Sep;:2300315Read more>>
- Tzu-Hsiang Lin. et al. A bioactive composite scaffold enhances osteochondral repair by using thermosensitive chitosan hydrogel and endothelial lineage cell-derived chondrogenic cell. MATER TODAY BIO. 2024 Oct;28:101174Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-CD144/VECD/VE Cadherin Polyclonal Antibody, Unconjugated (bs-0878R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
A549 lyates probed with Rabbit Anti-VE Cadherin Polyclonal Antibody, Unconjugated (bs-0878R) at 1:300 in 4˚C overnight. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.
Image provided by One World Lab validation program. HEK293 cells probed with Rabbit Anti-VE Cadherin Polyclonal Antibody (bs-0878R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-VE Cadherin Polyclonal Antibody (bs-0878R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Mouse heart lysates probed with VE Cadherin Polyclonal Antibody, unconjugated (bs-0878R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Images provided by the Independent Validation Program, badge number 029590:Formalin-fixed and frozen-OCT embedded mouse liver labeled with Rabbit Anti-VE Cadherin Polyclonal Antibody, Unconjugated(bs-0878R) 1:100, overnight at 4°C, The secondary antibody was diluted to 1:100 for 120 minutes at 37°C.
Mouse kidney lysates probed with VE Cadherin Polyclonal Antibody, Unconjugated (bs-0878R) at 3ug for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
RSC96 cells probed with VE Cadherin Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated (bs-0878R-A488) at 1:100 for 30 minutes compared to control cells (blue) and isotype control (orange).
HUVEC cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD144/VE Cadherin Polyclonal Antibody(bs-0878R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HUVEC cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD144/VE Cadherin Polyclonal Antibody(bs-0878R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).