bs-0830R [Primary Antibody]
Oct-4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: 4-Oct

Immunogen Range: 201-300/360


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5460

Source: KLH conjugated synthetic peptide derived from human Oct-4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 39


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen Z et al. Generation of an iPSC line (SKLOi001-A) from a patient with CLCN2-related leukoencephalopathy. Stem Cell Res. 2020 Mar 17;45:101769. Read more>>
  • Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/4 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93.Read more>>
  • Liu K et al. Verteporfin suppresses the proliferation, epithelial-mesenchymal transition and stemness of head and neck squamous carcinoma cells via inhibiting YAP1. Journal of Cancer. 2019; 10(18): 4196-4207. Read more>>
  • Li X et al. A comparative study of the behavior of neural progenitor cells in extrusion-based in vitro hydrogel models. Biomed Mater. 2019 Sep 9;14(6):065001. Read more>>
  • Sun Z et al. Glioblastoma Stem Cell-Derived Exosomes Enhance Stemness and Tumorigenicity of Glioma Cells by Transferring Notch1 Protein. Cell Mol Neurobiol. 2019 Dec 18. Read more>>
  • Chen Z et al. Generation of an iPSC line (SKLOi001-A) from a patient with CLCN2-related leukoencephalopathy. Stem Cell Res. 2020 Mar 17;45:101769. Read more>>
  • Wenjia Fang. et al. Prognostic value of OCT4 in colorectal cancer: analysis using immunohistochemistry and bioinformatics validation. 2020 Oct 14Read more>>
  • Xiong Xiao. et al. Construction of extracellular matrix-based 3D hydrogel and its effects on cardiomyocytes. Exp Cell Res. 2021 Nov;408:112843Read more>>
  • Que, Tianshi. et al. HMGA1 stimulates MYH9-dependent ubiquitination of GSK-3 via PI3K/Akt/c-Jun signaling to promote malignant progression and chemoresistance in gliomas. Cell Death Dis. 2021 Dec;12(12):1-12Read more>>
  • Lin Li. et al. Breast cancer stem cells-derived extracellular vesicles affect PPARG expression by delivering microRNA-197 in breast cancer cells. Clin Breast Cancer. 2022 FebRead more>>
  • Sijie Wang. et al. PFKFB4 facilitates palbociclib resistance in oestrogen receptor-positive breast cancer by enhancing stemness. CELL PROLIFERAT. 2022 Sep;:e13337Read more>>
  • Ping Xu. et al. Generation and characterization of two iPSC lines carrying heterozygous or homozygous nonsense mutation in PROM1 gene from a single family. STEM CELL RES. 2022 Sep;:102913Read more>>
  • Zou Yazhu. et al. Extracellular vesicles carrying miR-6836 derived from resistant tumor cells transfer cisplatin resistance of epithelial ovarian cancer via DLG2-YAP1 signaling pathway. INT J BIOL SCI. 2023 Jun;19(10):3099-3114Read more>>
  • Ping Xu. et al. Generation and characterization of two induced pluripotent stem cell lines from conjunctiva of a retinoblastoma patient. STEM CELL RES. 2023 Sep;:1032Read more>>
  • Yue Wu. et al. The biological characteristics of chicken embryo mesenchymal stem cells isolated from chorioallantoic membrane. GENESIS. 2024 Apr;62(2):e23592Read more>>
  • Zitong Zhao. et al. CircMALAT1 promotes cancer stem-like properties and chemoresistance via regulating Musashi-2/c-Myc axis in esophageal squamous cell carcinoma. MedComm. 2024 Jun;5(6):e612Read more>>
VALIDATION IMAGES

L1 rat brain, L2 , human colon carcinoma lysates probed (bs-0830R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 41kDa.


Formalin-fixed and paraffin embedded Human osteosarcoma labeled with Rabbit Anti-Oct-4/POU5F1 Polyclonal Antibody, Unconjugated (bs-0830R) 1:300 followed by conjugation to the secondary antibody and DAB staining.


Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Heart lysates; Lane 3: Mouse Cerebellum lysates; Lane 4: Mouse Testis lysates; Lane 5: Rat Cerebrum lysates; Lane 6: Rat Heart lysates; Lane 7: Rat Cerebellum lysates probed with Oct4 Polyclonal Antibody, Unconjugated (bs-0830R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.