Host: Rabbit
Target Protein: beta lactoglobulin
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 280838
Swiss Prot: P02754
Source: Beta-lactoglobulin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 18
Cross Reactive Species:
Sheep
Goat
Cow
For research use only. Not intended for diagnostic or therapeutic use.
- Zhong J et al.Comparison of antigenicity and conformational changes to β-lactoglobulin following kestose glycation reaction with and without dynamic high-pressure microfluidization treatment.(2019)Food Chem. Apr 25;278:491-496. Read more>>
- Yan-Hong Shaoet al. Glycation of -lactoglobulin combined by sonication pretreatment reduce its allergenic potential. Int J Biol Macromol. 2020 Dec 1;164:1527-1535.Read more>>
- Dan Bu. et al. Insight into the mechanism of d-allose in reducing the allergenicity and digestibility of ultrasound-pretreated -lactalbumin by high-resolution mass spectrometry. Food Chem. 2021 Nov;:131616Read more>>
Ewe's milk probed with Anti Beta-lactoglobulin Polyclonal Antibody, Unconjugated (bs-0814R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 19kD. Observed band size: 19kD
Paraformaldehyde-fixed, paraffin embedded (Sheep breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta lactoglobulin) Polyclonal Antibody, Unconjugated (bs-0814R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.