bs-0812R [Primary Antibody]
IL-1 Beta Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IL-1 Beta

Specificity: Predicted to work with Mouse and Rat samples, but with lower affinity.

Immunogen Range: 101-200/269


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3553

Swiss Prot: P01584

Source: KLH conjugated synthetic peptide derived from human IL-1beta

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
dot-blot()

Predicted Molecular Weight: 17/32


Cross Reactive Species: Human
Mouse
Rat
Bovine
Pig
Rabbit
Others

Predicted Cross Reactive Species: Dog
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhong, Shigen, et al. "Enhanced homing of mesenchymal stem cells to the ischemic myocardium by ultrasound-targeted microbubble destruction." Ultrasonics 52.2 (2012): 281-286.Read more>>
  • Bagriyanik, H. A., et al. "The effects of resveratrol on chronic constriction injury of sciatic nerve in rats." Neuroscience Letters (2014).Read more>>
  • Wang, Hui, et al. "Anti-inflammatory cytokine TSG-6 inhibits hypertrophic scar formation in a rabbit ear model." European Journal of Pharmacology (2015).Read more>>
  • Li, Kang, et al. "CD14 knockdown reduces lipopolysaccharide-induced cell viability and expression of inflammation-associated genes in gastric cancer cells in vitro and in nude mouse xenografts." Molecular Medicine Reports.Read more>>
  • Johann, Sonja, et al. "NLRP3 inflammasome is expressed by astrocytes in the SOD1 mouse model of ALS and in human sporadic ALS patients." Glia (2015).Read more>>
  • de Jesus, Julio Fernandes, et al. "Low-level laser therapy in IL-1β, COX-2, and PGE2 modulation in partially injured Achilles tendon." Lasers in Medical Science 30.1 (2015): 153-158.Read more>>
  • Hu, Ruiqi, et al. "Immunoreactivities of IL-1β and IL-1R in oviduct of Chinese brown frog (Rana dybowskii) during pre-hibernation and the breeding period."Acta histochemica 118.2 (2016): 164-169.Read more>>
  • Hu, Ruiqi, et al. "Immunoreactivities of IL-1β and IL-1R in oviduct of Chinese brown frog (Rana dybowskii) during pre-hibernation and the breeding period."Acta histochemica 118.2 (2016): 164-169.Read more>>
  • Xi, Liqin, et al. "Immunoreactivities of NF-κB, IL-1β and IL-1R in the skin of Chinese brown frog (Rana dybowskii)." Acta Histochemica (2016).Read more>>
  • Heitzer, Marius, et al. "Administration of 17β-Estradiol Improves Motoneuron Survival and Down-regulates Inflammasome Activation in Male SOD1 (G93A) ALS Mice." Molecular Neurobiology: 1-15.Read more>>
  • Liu, Dong-qing, Xiao-jing Li, and Xiao-juan Weng. "Effect of BTXA on Inhibiting Hypertrophic Scar Formation in a Rabbit Ear Model." Aesthetic Plastic Surgery (2017): 1-8.Read more>>
  • Du, Jinghua, et al. "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." Molecular medicine reports 13.3 (2016): 2159-2166.Read more>>
  • Liu et al. Autophagy induced by DAMPs facilitates the inflammation response in lungs undergoing ischemia-reperfusion injury through promoting TRAF6 ubiquitination. (2017) Cell.Death.Diffe. 24:683-693Read more>>
  • Han et al. Electroacupuncture modulated the inflammatory reaction in MCAO rats via inhibiting the TLR4/NF-κB signaling pathway in microglia. (2015) Int.J.Clin.Exp.Patho. 8:11199-205Read more>>
  • Ding et al. Porphyromonas gingivalis , a periodontitis causing bacterium, induces memory impairment and age-dependent neuroinflammation in mice. (2018) Immun.Ageing. 15:6Read more>>
  • Wei LY et al.Reciprocal activation of cancer-associated fibroblasts and oral squamous carcinoma cells through CXCL1.(2019)Oral Oncology.88:115-123.Read more>>
  • Chen S et al. Anthocyanins from Lycium ruthenicum Murr. ameliorated D-galactose-induced memory impairment, oxidative stress, and neuroinflammation in adult rats.J Agric Food Chem. 2019 Mar 20;67(11):3140-3149.Read more>>
  • Bian X et al. Periostin contributes to renal and cardiac dysfunction in rats with chronic kidney disease: reduction of PPARα.Biochimie. 2019 May;160:172-182.Read more>>
  • Liu W et al. Suppressive effect of glycyrrhizic acid against lipopolysaccharide-induced neuroinflammation and cognitive impairment in C57 mice via toll-like receptor 4 signaling pathway. Food Nutr Res. 2019 Apr 29;63.Read more>>
  • Li C et al. UFL1 modulates NLRP3 inflammasome activation and protects against pyroptosis in LPS-stimulated bovine mammary epithelial cells. Mol Immunol. 2019 May 8;112:1-9. Read more>>
  • Qu L et al. Glycyrrhizic acid ameliorates LPS-induced acute lung injury by regulating autophagy through the PI3K/AKT/mTOR pathway. Am J Transl Res. 2019 Apr 15;11(4):2042-2055.Read more>>
  • Dong Y et al. Red yeast rice ameliorates high-fat diet-induced atherosclerosis in Apoe−/− mice in association with improved inflammation and altered gut microbiota composition. Food Funct. 2019 Jun 12.Read more>>
  • Jiang JQ et al. Inhibition of LOX-1 alleviates the proinflammatory effects of high-mobility group box 1 in Aspergillus fumigatus keratitis. Int J Ophthalmol. 2019 Jun 18;12(6):898-903. Read more>>
  • Liu W et al. Synergistic effect of tolfenamic acid and glycyrrhizic acid on TPA-induced skin inflammation in mice. MedChemComm.2019.Read more>>
  • Zhang J et al. Bradykinin contributes to immune liver injury via B2R receptor-mediated pathways in trichloroethylene sensitized mice: a role in Kupffer cell activation. Toxicology. 2019 Mar 1;415:37-48.Read more>>
  • Zhang J et al. Bradykinin contributes to immune liver injury via B2R receptor-mediated pathways in trichloroethylene sensitized mice: a role in Kupffer cell activation. Toxicology. 2019 Mar 1;415:37-48.Read more>>
  • Huang H et al. The Function of PPARγ/AMPK/SIRT-1 Pathway in Inflammatory Response of Human Articular Chondrocytes Stimulated by Advanced Glycation End Products. Biol Pharm Bull. 2019;42(8):1303-1309. Read more>>
  • Wang B et al. Anti‐Adhesive, Platelet Gathering Effects of c‐RGD Modified Poly (p‐dioxanone‐co‐l‐Phe) Electrospun Membrane and Its Comprehensive Application in Intestinal Anastomosis. Macromol Biosci. 2019 Dec 18:e1900344.Read more>>
  • Jones LB et al. Effects of Pseudomonas aeruginosa on Microglial-Derived Extracellular Vesicle Biogenesis and Composition. Pathogens. 2019 Dec 14;8(4). pii: E297. Read more>>
  • Chen S et al. Anthocyanins from Lycium ruthenicum Murr. ameliorated D-galactose-induced memory impairment, oxidative stress, and neuroinflammation in adult rats.J Agric Food Chem. 2019 Mar 20;67(11):3140-3149.Read more>>
  • Gao M et al. Is NLRP3 or NLRP6 inflammasome activation associated with inflammation-related lung tumorigenesis induced by benzo (a) pyrene and lipopolysaccharide? Ecotoxicol Environ Saf. 2019 Sep 24;185:109687. Read more>>
  • Qiu R et al. 20 (S)-Ginsenoside Rg3-loaded electrospun membranes to prevent postoperative peritoneal adhesion. Biomed Microdevices. 2019 Aug 14;21(4):78. Read more>>
  • Chen JW et al. Zhihuang Tongfeng decoction ameliorates gouty arthritis via inhibition of NLRP3 inflammasome in rats. Tropical Journal of Pharmaceutical Research December 2019; 18 (12): 2577-2582.Read more>>
  • "Kang K et al. Dexmedetomidine protects against lipopolysaccharide-induced sepsis-associated acute kidney injury via an α7 nAChR-dependent pathway.Biomed Pharmacother. 2018 Oct;106:210-216. "Read more>>
  • "Murata K et al. Restoring knee joint kinematics after anterior cruciate ligament injury might inhibit synovial membrane inflammation.Sport Sciences for Health. 2018."Read more>>
  • Sun ZM et al. Resveratrol protects against CIH-induced myocardial injury by targeting Nrf2 and blocking NLRP3 inflammasome activation. Life Sci. 2020 Mar 15;245:117362.Read more>>
  • Zhang X et al. Drp1, a potential therapeutic target for Parkinson's disease, is involved in olfactory bulb pathological alteration in the Rotenone-induced rat model. Toxicol Lett. 2020 Feb 20;325:1-13.Read more>>
  • Kong D et al. IL-37 Gene Modification Enhances the Protective Effects of Mesenchymal Stromal Cells on Intestinal Ischemia Reperfusion Injury. Research Square. 17 February 2020.Read more>>
  • Seol BG et al. Skate cartilage extracts containing chondroitin sulfate ameliorates hyperlipidemia-induced inflammation and oxidative stress in high cholesterol diet-fed LDL receptor knockout mice in comparison with shark chondroitin sulfate. Nutr Res Pract. 2020 Jan;14:e3.Read more>>
  • Jin Z et al. Geniposide alleviates atherosclerosis by regulating macrophage polarization via the FOS/MAPK signaling pathway. Biomed Pharmacother. 2020 May;125:110015. Read more>>
  • Ji YR et al. Dexmedetomidine inhibits the invasion, migration, and inflammation of rheumatoid arthritis fibroblast-like synoviocytes by reducing the expression of NLRC5. Int Immunopharmacol. 2020 Mar 9;82:106374.Read more>>
  • Guan X et al. Estrogen deficiency aggravates apical periodontitis by regulating NLRP3/caspase-1/IL-1β axis. Am J Transl Res. 2020 Feb 15;12(2):660-671.Read more>>
  • Shao H et al. Glyburide attenuates ozone‐induced pulmonary inflammation and injury by blocking the NLRP3 inflammasome. Environ Toxicol. 2020 Mar 13.Read more>>
  • Yang F et al. Copper induces oxidative stress with triggered NF-B pathway leading to inflammatory responses in immune organs of chicken. Ecotoxicol Environ Saf. 2020 Sep 1;200:110715.Read more>>
  • Mengli Cui et al. NLRP3 inflammasome is involved in nerve recovery after sciatic nerve injury. Int Immunopharmacol. 2020 Jul;84:106492.Read more>>
  • Zhang Q et al. Transcriptome Analysis of the Gene Expression Profiles Associated with Fungal Keratitis in Mice Based on RNA-Seq. Invest Ophthalmol Vis Sci. 2020 Jun 3;61(6):32.Read more>>
  • Luo Q et al. Iron Overload Resulting from the Chronic Oral Administration of Ferric Citrate Impairs Intestinal Immune and Barrier in Mice. Biol Trace Elem Res. 2020 May 28.Read more>>
  • Shu-Min Yeet al. Silencing of Gasdermin D by siRNA-Loaded PEI-Chol Lipopolymers Potently Relieves Acute Gouty Arthritis through Inhibiting Pyroptosis. Mol Pharm. 2020 Jul 6.Read more>>
  • Chen Y et al. A selected small molecule prevents inflammatory osteolysis through restraining osteoclastogenesis by modulating PTEN activityClin Transl Med.2020 Dec;10(8):e240.Read more>>
  • Wang C et al. Lysyl oxidase suppresses the inflammatory response in anterior cruciate ligament fibroblasts and promotes tissue regeneration by targeting myotrophin via the nuclear factor-kappa B pathway. J Tissue Eng Regen Med. 2020 Aug;14(8):1063-1076.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse lung labeled with (bs-0812R) Rabbit Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated followed by conjugation to the secondary antibody and DAB staining.


Image was kindly submitted by Dr.Rachel Reith, NIH. Paraffin-embedded mouse brain stained with Rabbit Anti-IL-1 Beta Polyclonal Antibody,1:100 overnight at 4C.\n


Raw264.7 lysates probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-0812R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin-embedded Human Cervical Carcinoma; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4°C. Followed by incubation with the secondary antibody, DAB and counterstaining.


Paraformaldehyde-fixed, paraffin-embedded Rat Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4°C. Followed by incubation with a secondary antibody, DAB and counterstaining.


Paraformaldehyde-fixed, paraffin-embedded Rat Lung tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4°C. Followed by incubation with a secondary antibody, DAB and counterstaining.


Lane 1: Normal Mouse Raw264.7 cell lysates; Lane 2: Raw264.7 treated with 100ng/ml lipopolysaccharide (LPS) for 6h, then with 300ng/ml Brefeldin A added after 3 hours probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-0812R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.