Host: Rabbit
Target Protein: Collagen 4
Specificity: This antibody has 79% cross reactivity with Collagen alpha-5(IV) chain.
Immunogen Range: 1401-1500/1669
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 12826
Swiss Prot: P02463
Source: KLH conjugated synthetic peptide derived from mouse Collagen alpha-1(IV) chain
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 165
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
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Formalin-fixed and paraffin embedded rat liver tissue with Anti-Collagen IV Polyclonal Antibody, Unconjugated (bs-0806R) at 1:200, followed by conjugation to the secondary antibody and DAB staining\n
Formalin-fixed and paraffin embedded rabbit cartilage tissue with Anti-Collagen IV Polyclonal Antibody, Unconjugated (bs-0806R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Recombinant human Collagen IV protein, N-His (bs-42306P) probed with Collagen IV Polyclonal Antibody, Unconjugated (bs-0806R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.