bs-0793R [Primary Antibody]
RSL1D1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: RSL1D1

Immunogen Range: 151-250/490


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 26156

Swiss Prot: O76021

Source: KLH conjugated synthetic peptide derived from human CSIG/RSL1D1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Regulates cellular senescence through inhibition of PTEN translation. Acts as a pro-apoptotic regulator in response to DNA damage.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 55


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li, Xiao‑Ping, et al. "Overexpression of ribosomal L1 domain containing 1 is associated with an aggressive phenotype and a poor prognosis in patients with prostate cancer." Oncology Letters.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-CSIG Polyclonal Antibody, Unconjugated (bs-0793R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


L1 rat brain lysates L2 rat kidney lysates probed with Anti CSIG Polyclonal Antibody, Unconjugated (bs-0793R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 54-60kD. Observed band size:54kD.\n


Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RSL1D1) Polyclonal Antibody, Unconjugated (bs-0793R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.