bs-0782R [Primary Antibody]
IL-6 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IL-6

Immunogen Range: 111-150/211


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 16193

Swiss Prot: P08505

Source: KLH conjugated synthetic peptide derived from mouse IL-6

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 23


Cross Reactive Species: Human
Mouse
Rat
Bovine
Rabbit
Others

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sun, Chao, et al. "Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue." BioMed Research International 2015 (2015).Read more>>
  • Taskin, Mine Islimye, et al. "Prevention of ovarian hyperstimulation syndrome in a rat model: comparison of the efficacy of tocilizumab with that of ranibizumab, cabergoline, and a gonadotropin-releasing hormone antagonist."Gynecological Endocrinology (2015): 1-6.Read more>>
  • Taskin, Mine Islimye, et al. "A Humanized Anti-Interleukin 6 Receptor Monoclonal Antibody, Tocilizumab, for the Treatment of Endometriosis in a Rat Model." Reproductive Sciences (2015): 1933719115612134.Read more>>
  • Kuracha, Murali R., et al. "Patient‐derived xenograft mouse models of pseudomyxoma peritonei recapitulate the human inflammatory tumor microenvironment." Cancer Medicine (2016).Read more>>
  • Takayanagi, Takehiko, et al. "Caveolin 1 is critical for abdominal aortic aneurysm formation induced by angiotensin II and inhibition of lysyl oxidase." Clinical Science 126.11 (2014): 785-800.Read more>>
  • Haffner-Luntzer, Melanie, et al. "The inflammatory phase of fracture healing is influenced by oestrogen status in mice." European Journal of Medical Research 22.1 (2017): 23.Read more>>
  • Prystaz, Katja, et al. "Distinct effects of interleukin-6 classic and trans-signaling in bone fracture healing." The American Journal of Pathology.Read more>>
  • Luo et al. Maternal Bisphenol A Diet Induces Anxiety-like Behavior in Female Juvenile with Neuroimmune Activation. (2014) Toxicol.Sc. Read more>>
  • Luo et al. Maternal Bisphenol A Diet Induces Anxiety-like Behavior in Female Juvenile with Neuroimmune Activation. (2014) Toxicol.Sc. Read more>>
  • Zhu et al. Hematopoietic Effects of Paeoniflorin and Albiflorin on Radiotherapy-Induced Myelosuppression Mice. (2016) Evid.Based.Complement.Alternat.Me. 2016:5789381Read more>>
  • Chang et al. Interleukin-6 (IL-6) mediated the increased contraction of distal colon in streptozotocin-induced diabetes in rats via IL-6 receptor pathway. (2015) Int.J.Clin.Exp.Patho. 8:4514-24Read more>>
  • Sun et al. Neuroprotection by IFN-? via astrocyte-secreted IL-6 in acute neuroinflammation. (2017) Oncotarget. 8:40065-40078Read more>>
  • Han et al. Anti-inflammatory effect of polydeoxyribonucleotide on zoledronic acid-pretreated and lipopolysaccharide-stimulated RAW 264.7 cells. (2018) Exp.Ther.Med. 16:400-405Read more>>
  • Guo,et al.Establishment of an immortalized mouse dermal papilla cell strain with optimized culture strategy.(2018) PeerJ. 6:e4306.Read more>>
  • Bai YH et al. LncRNA NEAT1 promotes inflammatory response and induces corneal neovascularization.（ 2018） J Mol Endocrinol. Oct 15;61(4):231-239Read more>>
  • Saggese T et al. GEORG SCHMORL PRIZE OF THE GERMAN SPINE SOCIETY (DWG) 2018: combined inflammatory and mechanical stress weakens the annulus fibrosus: evidences from a loaded bovine AF organ culture. Eur Spine J. 2019 Jan 28.Read more>>
  • Li B et al. Increased hepcidin in hemorrhagic plaques correlates with iron-stimulated IL-6/STAT3 pathway activation in macrophages. Biochem Biophys Res Commun. 2019 Jul 23;515(2):394-400. Read more>>
  • Liu W et al. Synergistic effect of tolfenamic acid and glycyrrhizic acid on TPA-induced skin inflammation in mice. MedChemComm.2019.Read more>>
  • Afdal A et al. The Expression of Transforming Growth Factor Beta-1 and Interleukin-6 on Human Prostate: Prostate Hyperplasia and Prostate Cancer. Open Access Maced J Med Sci. 2019 Jun 30;7(12):1905-1910.Read more>>
  • Yuan SJ et al. Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer.J Nanobiotechnology. 2019 Oct 17;17(1):110. Read more>>
  • Gao M et al. Activating the interleukin-6-Gp130-STAT3 pathway ameliorates ventricular electrical stability in myocardial infarction rats by modulating neurotransmitters in the paraventricular nucleus. BMC Cardiovasc Disord. 2020 Feb 5;20(1):60.Read more>>
  • Ahmed S et al. Cocoa Flavonoids Reduce Inflammation and Oxidative Stress in a Myocardial Ischemia-Reperfusion Experimental Model. Antioxidants (Basel). 2020 Feb 18;9(2). pii: E167.Read more>>
  • Jin W et al. Tannic acid ameliorates arsenic trioxide-induced nephrotoxicity, contribution of NF-κB and Nrf2 pathways. Biomed Pharmacother. 2020 Jun;126:110047. Read more>>
  • Ji YR et al. Dexmedetomidine inhibits the invasion, migration, and inflammation of rheumatoid arthritis fibroblast-like synoviocytes by reducing the expression of NLRC5. Int Immunopharmacol. 2020 Mar 9;82:106374.Read more>>
  • Edi Simoni et al. mouse. Front Neurol. 2020 May 14;11:341.Read more>>
  • Zhang Q et al. Transcriptome Analysis of the Gene Expression Profiles Associated with Fungal Keratitis in Mice Based on RNA-Seq. Invest Ophthalmol Vis Sci. 2020 Jun 3;61(6):32.Read more>>
  • Shuang Huet al. MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1. Life Sci. 2020 Oct 1;258:118147.Read more>>
  • Yan Li. et al. Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs. Sci Adv. 2021 Feb;7(9):eabd674Read more>>
  • Haile Liu. et al. Catalytically potent and selective clusterzymes for modulation of neuroinflammation through single-atom substitutions. Nat Commun. 2021 Jan;12(1):1-14Read more>>
  • Hongyi Qu. et al. The effects of vasoactive intestinal peptide on RANKL-induced osteoclast formation. Ann Transl Med. 2021 Jan; 9(2): 127Read more>>
  • Lu Xue. et al. A novel peptide relieves endothelial cell dysfunction in?preeclampsia by regulating the PI3K/mTOR/HIF1 pathway. Int J Mol Med. 2021 Jan;47(1):276-288Read more>>
  • Zhenting Huang . et al. Anti-Cognitive Decline by Yinxing-Mihuan-Oral-Liquid via Activating CREB/BDNF Signaling and Inhibiting Neuroinflammatory Process. 2021 Jan 27Read more>>
  • Li-You Chen. et al. Effects of Rutin on Wound Healing in Hyperglycemic Rats. Antioxidants-Basel. 2020 Nov;9(11):1122Read more>>
  • Yingran Liang. et al. Crocin ameliorates arsenic trioxide?induced cardiotoxicity via Keap1-Nrf2/HO-1 pathway: Reducing oxidative stress, inflammation, and apoptosis. Biomed Pharmacother. 2020 Nov;131:110713Read more>>
  • Siqi Ying. et al. Low-intensity Pulsed Ultrasound regulates alveolar bone homeostasis in experimental Periodontitis by diminishing Oxidative Stress. Theranostics. 2020; 10(21): 9789C9807Read more>>
  • Shuangshuang Li. et al. Inhibiting Rab27a in renal tubular epithelial cells attenuates the inflammation of diabetic kidney disease through the miR-26a-5p/CHAC1/NF-kB pathway. Life Sci. 2020 Nov;261:118347Read more>>
  • Xiaoxuan Lei. et al. Human Salivary Histatin-1 Is More Efficacious in Promoting Acute Skin Wound Healing Than Acellular Dermal Matrix Paste. Front Bioeng Biotech. 2020; 8: 999Read more>>
  • Tianyan Chen. et al. Human Neural Stem Cell-Conditioned Medium Inhibits Inflammation in Macrophages Via Sirt-1 Signaling Pathway In Vitro and Promotes Sciatic Nerve Injury Recovery in Rats. Stem Cells Dev. 2020 Aug;29(16):1084-1095Read more>>
  • Xin Chen. et al. Aurka loss in CD19+ B cells promotes megakaryocytopoiesis via IL-6/STAT3 signaling-mediated thrombopoietin production. Theranostics. 2021; 11(10): 4655C4671Read more>>
  • Ye Han. et al. Pro\inflammatory cytokine interleukin\6\induced hepcidin, a key mediator of periodontitis\related anemia of inflammation. 2021 Mar 03Read more>>
  • in Yu. et al. CIHH protects the heart against left ventricular remodelling and myocardial fibrosis by balancing the renin-angiotensin system in SHR. Life Sci. 2021 Aug;278:11954Read more>>
  • in Feng. et al. Li-ESWT treatment reduces inflammation, oxidative stress, and pain via the PI3K/AKT/FOXO1 pathway in autoimmune prostatitis rat models. 2021 May 07Read more>>
  • Hui Shi. et al. Astragaloside IV prevents acute myocardial infarction by inhibiting the TLR4/MyD88/NF-B signaling pathway. 2021 May 25Read more>>
  • Wenhui Huang. et al. Mechanical stretching of the pulmonary vein mediates pulmonary hypertension due to left heart disease by regulating SAC/MAPK pathway and the expression of IL-6 and TNF-. J Cardiothorac Surg. 2021 Dec;16(1):1-12Read more>>
  • Ya-nan Fu. et al. Spatiotemporally dynamic therapy with shape-adaptive drug-gel for the improvement of tissue regeneration with ordered structure. Bioact Mater. 2021 JuRead more>>
  • Yan Li. et al. Salicylic acid-based nanomedicine with self-immunomodulatory activity facilitates microRNA therapy for metabolic skeletal disorders. Acta Biomater. 2021 JuRead more>>
  • Li-You Chen. et al. Therapeutic Potential of Luteolin on Impaired Wound Healing in Streptozotocin-Induced Rats. Biomedicines. 2021 Jul;9(7):761Read more>>
  • Sher Bahadur Poudel. et al. Sexual dimorphic impact of adult-onset somatopause on life span and age-induced osteoarthritis. 2021 Jul 09Read more>>
  • Quan-Kuo He. et al. Captan exposure disrupts ovarian homeostasis and affects oocytes quality via mitochondrial dysfunction induced apoptosis. Chemosphere. 2022 Jan;286:131625Read more>>
  • Yuan SJ. et al. Conjugation with nanodiamonds via hydrazone bond fundamentally alters intracellular distribution and activity of doxorubicin.. Int J Pharmaceut. 2021 Jul;606:120872-120872Read more>>
VALIDATION IMAGES

L1 mouse lung lysates L2 protein provided by the end user lysates probed with Anti IL-6 Polyclonal Antibody, Unconjugated (bs-0782R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 23kD. Observed band size:23kD.


Formalin-fixed and paraffin embedded mouse lung tissue labeled with Anti-IL-6(mo, rat) Polyclonal Antibody, Unconjugated (bs-0782R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-IL-6(mo, rat) Polyclonal Antibody, Unconjugated (bs-0782R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


HUVEC (H) cells were treated with or without LPS (0.5 μg/ml，24h) and Brefeldin A (300 ng/ml，20h) , 25 μg total protein per lane of cell lysates (see on figure) probed with IL-6 polyclonal antibody, unconjugated (bs-0782R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.