bs-0774R
[Primary Antibody]
Mouse IgA Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Mouse IgA
Clonality: Polyclonal
Isotype: IgG
Source: Mouse IgA protein purified from serum
Purification: affinity Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Mouse IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain-linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids, and milk. Mucosal IgA plays a major role in host defense by neutralizing infectious agents at mucosal surfaces. The production is usually local and antigen-specific IgA-producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA-producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal-produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF()
Cross Reactive Species: Mouse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Dong, Yulan, et al. "Effects of Chemical Sympathectomy on the Level of Reproductive Hormones and Mast Cells During Mouse Early Pregnancy." Asian Journal of Animal and Veterinary Advances 7.10 (2012): 960-969.Read more>>
- Yao L et al. The proteasome activator REGγ counteracts immunoproteasome expression and autoimmunity. J Autoimmun. 2019 Jun 3. Read more>>
- in Zeng. et al. Porcine Milk-Derived Small Extracellular Vesicles Promote Intestinal Immunoglobulin Production through pIgR. Animals-Basel. 2021 Jun;11(6):1522Read more>>
- Yanqiao Wen. et al. Regulation of Yujin Powder alcoholic extracts on ILC3s-TD IgA-colonic mucosal flora axis of DSS-induced ulcerative colitis.. FRONT MICROBIOL. 2022 Oct;13:1039884-1039884Read more>>
- Hao An. et al. placenta-specific 8 facilitates the infection of duck hepatitis A virus type 1 by inhibiting the TLR7 MyD88-dependent signaling pathway. POULTRY SCIENCE. 2023 Apr;:102724Read more>>
- An Hao. et al. Interleukin-22 facilitates the interferon--mediated production of tripartite motif protein 25 to inhibit replication of duck viral hepatitis A virus type 1. VET RES. 2023 Dec;54(1):1-13Read more>>
- Zhibo Zeng. et al. Lactobacillus plantarum modulate gut microbiota and intestinal immunity in cyclophosphamide-treated mice model. BIOMED PHARMACOTHER. 2023 Dec;169:115812Read more>>
- Di Chen. et al. Polysaccharide from Areca catechu L. inflorescence enhances the intestinal mucosal immunity to maintain immune homeostasis. INT J BIOL MACROMOL. 2024 Oct;278:1349Read more>>
VALIDATION IMAGES
Lane 1: Serum (Mouse) at 30 ug Lane 2: Plasma (Mouse) at 30 ug Lane 3: Liver (Mouse) Tissue Lysate at 40 ug Lane 4: Cerebrum (Mouse) Tissue Lysate at 40 ug Lane 5: Spleen (Mouse) Tissue Lysate at 40 ug Primary: Mouse IgA (bs-0774R-HRP) at 1/1000 dilution Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 62 kD
Lane 1: Serum (Mouse) at 40 ug Lane 2: Small intestine (Mouse) Lysate at 40 ug Lane 3: Lung (Mouse) Lysate at 40 ug Primary: Mouse IgA (bs-0774R) at 1/1000 dilution Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 60 kD