bs-0771R [Primary Antibody]
CaMK2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CaMK2

Specificity: The peptide immunogen is highly conserved between CaMKII alpha, beta, gamma, and delta.

Immunogen Range: 101-200/478


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 816

Swiss Prot: Q13554

Source: KLH conjugated synthetic peptide derived from human CaMK2 alpha

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)

Predicted Molecular Weight: 54


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Tang Q et al. Aqueous extract from You-Gui-Yin ameliorates cognitive impairment of chronic renal failure micethrough targeting hippocampal CaMKIIα/CREB/BDNF and EPO/EPOR pathways. J Ethnopharmacol. 2019 Jul 15;239:111925. Read more>>
  • Hou Y et al. Longzhibu disease and its therapeutic effects by traditional Tibetan medicine: Ershi-wei Chenxiang pills. J Ethnopharmacol. 2019 Nov 24:112426. Read more>>
  • Di Xia et al. Nrf2 promotes esophageal squamous cell carcinoma (ESCC) resistance to radiotherapy through the CaMKII-associated activation of autophagy. Cell Biosci. 2020 Jul 30;10:90.Read more>>
  • Ling Chen. et al. HC067047 as a potent TRPV4 inhibitor repairs endotoxemia colonic injury. INT IMMUNOPHARMACOL. 2023 Mar;116:109648Read more>>
  • Jialun Yang. et al. Aerobic exercise regulates GPR81 signal pathway and mediates complement- microglia axis homeostasis on synaptic protection in the early stage of Alzheimer's disease. LIFE SCI. 2023 Oct;331:122042Read more>>
  • Hailin Liu. et al. Dexamethasone upregulates macrophage PIEZO1 via SGK1, suppressing inflammation and increasing ROS and apoptosis. BIOCHEM PHARMACOL. 2024 Apr;222:11605Read more>>
VALIDATION IMAGES

Mouse heart lysates probed with Rabbit Anti-CaMK2 Polyclonal Antibody, Unconjugated (bs-0771R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT


Mouse spleen cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CaMK2 Polyclonal Antibody (bs-0771R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Lane 1: Mouse Heart tissue lysates; Lane 2: Rat Heart tissue lysates probed with CaMK2 alpha Polyclonal Antibody, Unconjugated (bs-0771R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


25 ug total protein per lane of various lysates (see on figure) probed with CaMK2 alpha polyclonal antibody, unconjugated (bs-0771R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.